Hyperglycemia and reduced adiposity of streptozotocin-induced diabetic mice are not alleviated by oral benzylamine supplementation  

作　　者：Christian Carpéné Kristiyan Stiliyanov Atanasov Francisco Les Josep Mercader Barcelo 

机构地区：[1]Institut des Maladies Métaboliques et Cardiovasculaires,INSERM UMR 1297,Toulouse 31432,France [2]Molecular Biology and One Health research group,Department of Fundamental Biology and Health Sciences,University of the Balearic Islands,Palma 07122,Spain [3]Department of Pharmacy,Faculty of Health Sciences,Universidad San Jorge,Villanueva de Gállego,Zaragoza 50830,Spain

出　　处：《World Journal of Diabetes》2022年第9期752-764,共13页世界糖尿病杂志（英文版）（电子版）

摘　　要：BACKGROUND Benzylamine(Bza)oral administration delays the onset of hyperglycemia in insulin-resistant db-/-mice;a genetic model of obesity and type 2 diabetes.AIM To extend the antihyperglycemic properties of oral benzylamine to a model of insulin-deficient type 1 diabetes.METHODS Male Swiss mice were rendered diabetic by streptozotocin treatment(STZ)and divided in two groups:one received 0.5%Bza as drinking solution for 24 d(STZ Bza-drinking)while the other was drinking water ad libitum.Similar groups were constituted in age-matched,nondiabetic mice.Food intake,liquid intake,body weight gain and nonfasting blood glucose levels were followed during treatment.At the end of treatment,fasted glycemia,liver and white adipose tissue(WAT)mass were measured,while glucose uptake assays were performed in adipocytes.RESULTS STZ diabetic mice presented typical features of insulin-deficient diabetes:reduced body mass and increased blood glucose levels.These altered parameters were not normalized in the Bza-drinking group in spite of restored food and water intake.Bza consumption could not reverse the severe fat depot atrophy of STZ diabetic mice.In the nondiabetic mice,no difference was found between control and Bza-drinking mice for any parameter.In isolated adipocytes,hexose uptake was partially activated by 0.1 mmol/L Bza in a manner that was obliterated in vitro by the amine oxidase inhibitor phenelzine and that remained unchanged after Bza supplementation.Oxidation of 0.1 mmol/L Bza in WAT was lower in STZ diabetic than in normoglycemic mice.CONCLUSION Bza supplementation could not normalize the altered glucose handling of STZ diabetic mice with severe WAT atrophy.Consequently,its antidiabetic potential in obese and diabetic rodents does not apply to lipoatrophic type 1 diabetic mice.

关 键 词：Diabetes ADIPOCYTES Amine oxidases Insulin-like agents Glucose transport POLYDIPSIA 

分 类 号：R587.1[医药卫生—内分泌]