肝癌组织中miR-7-5p、UbcH10表达水平与患者临床预后的相关性研究  

Correlation between the expression levels of miR-7-5p and UbcH10 in tissues of liver cancer and clinical prognosis

作　　者：檀喜玲 冯喆 高芳 TAN Xiling;FENG Zhe;GAO Fang(Department of Gastroenterology,Handan People's Hospital,Handan,Hebei 056009,China;Department of General Surgery,Handan Central Hospital,Handan,Hebei 056001,China;Department of General Surgery,Handan First Hospital,Handan,Hebei 056002,China)

机构地区：[1]邯郸市人民医院消化内科,河北邯郸056009 [2]邯郸市中心医院普外科,河北邯郸056001 [3]邯郸市第一医院普外科,河北邯郸056002

出　　处：《国际检验医学杂志》2022年第17期2146-2151,共6页International Journal of Laboratory Medicine

基　　金：河北省医学科学研究课题(20200191)。

摘　　要：目的探讨原发性肝癌(简称肝癌)组织中微小RNA(miR)-7-5p、UbcH10表达水平与患者临床预后的相关性。方法回顾性分析2017年12月至2018年12月间在邯郸市人民医院行肝癌切除手术治疗的57例肝癌患者临床资料,术中分别采集肝癌组织和癌旁组织57份,采用荧光定量聚合酶链式反应(qRT-PCR)测定肝癌组织和癌旁组织中miR-7-5p及UbcH10的表达水平,分析癌组织中miR-7-5p及UbcH10表达水平与临床病理特征及预后的相关性。结果肝癌组织中miR-7-5p表达水平则明显低于癌旁组织,UbcH10表达水平显著高于癌旁组织,差异均有统计学意义(P<0.05);肝癌组织中miR-7-5p表达水平与分化程度、包膜形成、门静脉侵犯等特征相关(P<0.05);UbcH10相对表达与肿瘤最大径、分化程度、TNM分期、门静脉侵犯相关(P<0.05)。肝癌随访期无进展生存率为38.60%,miR-7-5p高表达患者无进展生存率为88.89%,miR-7-5p低表达患者无进展生存率为29.17%,差异有统计学意义(χ^(2)=12.390,P<0.001);UbcH10高表达患者无复发生存率(RFS)为52.50%,UbcH10低表达患者RFS为82.35%(χ^(2)=4.196,P=0.041)。结论肝癌组织中的miR-7-5p表达水平降低,而UbcH10表达水平升高,且均与肝癌病理特征及术后复发率密切相关,miR-7-5p及UbcH10可作为肝癌早期诊断和靶向治疗的生物分子标志物。Objective To investigate the correlation between the expression levels of miR-7-5 p and UbcH10 in the tissues of patients with primary liver cancer(referred to as liver cancer)and the clinical prognosis.Methods The clinical data of totally 57 patients with liver cancer who underwent liver cancer resection in Handan People′s Hospital from December 2017 to December 2018 were retrospectively analyzed,and 57 liver cancer tissues and adjacent cancer tissues were collected respectively during the eperation.The expression levels of miR-7-5 p and UbcH10 in the liver cancer tissues and adjacent cancer tissues were determined by Fluorescence quantitative polymerase chain reaction(qRT-PCR),and the correlation between the expression levels of miR-7-5 p and UbcH10 in cancer tissues and clinicopathological characteristics and prognosis was analyzed.Results The expression level of miR-7-5 p in the liver cancer tissue was significantly lower than that in adjacent cancer tissue,while the expression level of UbcH10 was significantly higher than that in adjacent cancer tissue,and the differences were statistically significant(P<0.05).The expression level of miR-7-5 p in the liver cancer tissues was correlated with differentiation degree,capsule formation and portal vein invasion(P<0.05).The expression level of UbcH10 was correlated with tumor maximum diameter,differentiation degree,TNM stage and portal vein invasion(P<0.05).The progression-free survival rate in the follow-up period of liver cancer was 38.60%,the progression-free survival rate of patients with high miR-7-5 p expression was 88.89%,the progression-free survival rate of patients with low miR-7-5 p expression was 29.17%,and the differences were statistically significant(χ^(2)=12.390,P<0.001).The recurrence-free survival rate(RFS)of patients with high UbcH10 expression was 52.50%,and the RFS of patients with low UbcH10 expression was 82.35%,and the differences were statistically significant(χ^(2)=4.196,P=0.041).Conclusion The expression level of miR-7-5 p in liver ca

关 键 词：原发性肝癌 微小RNA-7-5p UBCH10 病理特征 预后 

分 类 号：R735.7[医药卫生—肿瘤]