Analysis of invasiveness and tumor-associated macrophages infiltration in solid pseudopapillary tumors of pancreas  

作　　者：Jie Yang Chun-Lu Tan Dan Long Yan Liang Li Zhou Xu-Bao Liu Yong-Hua Chen 

机构地区：[1]Department of Pancreatic Surgery,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China [2]Key Laboratory of Transplant Engineering and Immunology of the Ministry of Health,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China [3]Core Facilities,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China

出　　处：《World Journal of Gastroenterology》2022年第34期5047-5057,共11页世界胃肠病学杂志（英文版）

基　　金：Supported by Natural Science Foundation of China,No. 82071746;Key Research and Development Projects in Sichuan Province,No. 2019YFS0043;1·3·5 Project for Disciplines of Excellence–Clinical Research Incubation Project,West China Hospital,Sichuan University,No. ZY2017302

摘　　要：BACKGROUND Solid pseudopapillary tumor(SPT)is a rare pancreatic tumor.Considering its malignant behaviors,SPT has been classified as a low-grade malignant tumor.Indeed,only 9.2%of all SPT patients are initially diagnosed as malignant with invasion or metastasis.Thus,one of the challenges in managing SPT patients is predicting malignant behavior.AIM To investigate the malignant behavior and tumor-associated macrophage(TAM)infiltration between different histopathologic features of SPT patients.METHODS Twenty-five formalin-fixed paraffin-embedded tissue samples from 22 patients pathologically diagnosed with an SPT between 2009 and 2019 at West China Hospital were included in this retrospective study.Integrity of the capsule and growth pattern of the tumor cells was assessed microscopically in hematoxylineosin(HE)-stained sections.Based on the histopathological features,the SPT patients were divided into two groups:capsule or invasion.Clinical features,malignant behavior,and TAM infiltration were compared between the two groups.RESULTS Among the 22 SPT patients,11 were identified for each group,having either a capsule or invasion histopathologic feature.Malignant behavior was more frequent in the invasion group,including 2 patients who had peripheral organ invasion,3 with liver metastasis,and 1 with both lymph node and spleen metastases(P=0.045).Ki-67 index of more than 3%was also more frequent in the invasion group(P=0.045).Immunohistochemical analysis showed that the invasion group had a significant increase of CD68-positive TAMs in intratumor and peritumor sites in comparison with the capsule group(all P<0.0001).Similarly,CD163-positive M2-like macrophages were also markedly increased in the intratumor and peritumor sites in the invasion group(all P<0.0001).At the liver metastasis site,both intratumor and peritumor tissues showed relatively high-level CD68-positive TAMs and CD163-positive M2-like macrophages infiltration.However,the differences between the intratumor,peritumor and normal hepatic tissues did not re

关 键 词：Pancreatic neoplasms Solid pseudopapillary tumors MALIGNANCY Tumor-associated macrophages Histological labeling IMMUNOHISTOCHEMISTRY 

分 类 号：R735.9[医药卫生—肿瘤]