汞胁迫对脊尾白虾肌肉组织损伤及免疫相关酶活性的影响  

Effects of Mercury Stress on Muscle Tissue Injury and Immune Related Enzyme Activities in Ridgetail White Prawn Exopalaemon carinicauda

作　　者：王林华 欧阳乐飞 高威 段健诚 张庆起 高焕 WANG Linhua;OUYANG Lefei;GAO Wei;DUAN Jiancheng;ZHANG Qingqi;GAO Huan(Jiangsu Key Laboratory of Marine Biotechnology,College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Academy of Marine Resources Development,Lianyungang 222001,China;Jiangsu Province’s Agricultural Germplasm Resource Protection and Utilization Platform,Nanjing 210014,China;Ganyu-jiaxin Aquaculture Linited Company of Lianyungang,Lianyungang 222100,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [2]江苏省海洋资源开发研究院,江苏连云港222001 [3]江苏省农业种质资源保护与利用平台,江苏南京210014 [4]连云港赣榆佳信水产开发有限公司,江苏连云港222100

出　　处：《水产学杂志》2022年第4期47-52,共6页Chinese Journal of Fisheries

基　　金：江苏省研究生科研与实践计划项目(SJCX19-0980);江苏省优势学科建设工程资助项目(PAPD);江苏海洋大学江苏省海洋生物资源与环境重点实验室开放课题(SH20191203).

摘　　要：在水温(25±1)℃下,将体长(4.5±0.5)cm的脊尾白虾(Exopalaemon carinicauda)放在60 L(50 cm×40 cm×30 cm)的蓝色水箱中,不换水,不投喂,在0 g/L(对照组)、1×10^(-6)g/L、3×10^(-6)g/L和1×10^(-5)g/L浓度的汞(Hg^(2+))胁迫0 h、6 h、12 h、24 h、48 h、72 h和96 h时,每组取3尾虾和0.2 g肌肉,先用液氮速冻,再放入-80℃保存,用于测定超氧化物歧化酶(SOD)活性;另取0.5 cm^(3)肌肉用Bouin’s液固定,用于组织切片,同时记录各组试验虾的中毒症状及死亡情况,研究了汞胁迫对虾肌纤维结构及肌肉组织中SOD活力的影响。结果表明,与对照组相比,汞胁迫组虾的肌纤维结构在前48 h内变化不明显;72 h和96 h时,肌纤维变化明显:低浓度汞胁迫(1×10^(-6)g/L)72 h肌纤维没有明显变化,胁迫96 h部分肌纤维溶解;高浓度汞胁迫(1×10^(-5)g/L)72 h,部分肌纤维溶解,胁迫96 h部分肌纤维断裂。不同浓度Hg^(2+)胁迫后,肌肉组织中SOD活力呈先下降后上升的趋势,在24 h时均达到最低值,但不同浓度胁迫组之间存在差异。低浓度胁迫组(1×10^(-6)g/L)的SOD活力在96 h达到最高,为24.639 U/mg;高浓度胁迫组(3×10^(-6)g/L和1×10^(-5)g/L)的SOD活力在72 h时达到最高,分别为21.744 U/mg和25.780 U/mg。这表明,汞胁迫对脊尾白虾代谢会产生影响,导致氧化应激,对脊尾白虾肌肉组织纤维造成一定损伤。In order to study the effects of mercury stress on the organism of ridgetail white prawn Exopalaemon carinicauda,the changes in muscle fiber structure and superoxide dismutase(SOD)activity were investigated in muscle tissue of ridgetail white prawn held in 60 L blue water tanks of each 50 cm×40 cm×30 cm exposed to mercury(Hg^(2+))concentrations of 1×10^(-6)g/L,3×10^(-6)g/L and 1×10^(-5)g/L at water temperature of(25±1)℃during 96 h stress(0 h,6 h,12 h,24 h,48 h,72 h and 96 h).The histological observation showed that the muscle fiber structure of the ridgetail white prawn exposed to mercury stress was not changed significantly in the first 48 h,but changed significantly from 72 h to 96 h compared with the control group.The structures of the muscle fiber were not shown to be changed significantly at the low concentration mercury(1×10^(-6)g/L)exposure for 72 h,and to be partially dissolved after stressed for 96 h.In contrast,the muscle fibers were partially dissolved at high concentration mercury(1×10^(-5)g/L)exposure for 72 h,and it was partially broken after stressed for 96 h.Moreover,after different concentrations of Hg^(2+)stress,there was a trend of first decline and then elevated SOD activity in muscle tissue,with the minimal value at 24 h,with differences among Hg^(2+)stress groups:the maximal SOD activity(24.639 U/mg)at low concentration stress group(1×10^(-6)g/L)at 96 h,and the maximal SOD activity at high concentration stress groups(21.744 U/mg in 3×10^(-6)g/L group and 25.780 U/mg in 1×10^(-5)g/L group)at 72 h.These findings illustrate that mercury stress will affect the metabolism of ridgetail white prawn and cause oxidative stress and certain damage to muscle fibers,which take a certain significance for revealing the toxic effect and mechanism of heavy metals on crustaceans.

关 键 词：脊尾白虾 汞胁迫 肌纤维 超氧化物歧化酶 

分 类 号：S917.4[农业科学—水产科学] X174[环境科学与工程—环境科学]