Alpha2-adrenergic receptor activation reinstates motor deficits in rats recovering from cortical injury  

作　　者：Gabriela García-Díaz Laura ERamos-Languren Carmen Parra-Cid Joel Lomelí Sergio Montes Camilo Ríos Antonio Bueno-Nava Ignacio Valencia-Hernández Rigoberto González-Piña 

机构地区：[1]Department of Pharmacology,Section of Postgraduate Studies and Research,High Medical School,Instituto Politécnico Nacional,Salvador Díaz Mirón,Alcaldía Miguel Hidalgo,Mexico City,Mexico [2]Department of Neurobiology of Aging and Brain Damage,Coordination of Psychobiology and Neurosciences,Faculty of Psychology,National Autonomous University of Mexico,Av.Universidad 3040,Col.Copilco Universidad,Alcaldía Coyoacán,Mexico City,Mexico [3]Direction of Research,National Institute of Rehabilitation LGII,Calz.Mexico-Xochimilco 289,Col.Arenal de Guadalupe,Alcaldía Tlalpan,Mexico City,Mexico [4]Department of Pharmacology,Multidisciplinary Academic Unit Reynosa-Aztlan Reynosa,Autonomous University of Tamaulipas,calle 16 y lago de Chapala,Col.Aztlán,Tamaulipas,Mexico [5]Department of Neurochemistry,National Institute of Neurology and Neurosurgery MVS,Av.Insurgentes Sur 3877,Col.La Fama,Alcaldia Tlalpan,Mexico City,Mexico [6]Department of Special Education,University of the Americas,Puebla 223,Col.Roma,Alcaldía Cuauhtémoc,Mexico City,Mexico

出　　处：《Neural Regeneration Research》2023年第4期875-880,共6页中国神经再生研究（英文版）

基　　金：supported by Consejo Nacional de Ciencia y Tecnología(CONACy T)project CB 2016-287614(to RGP and ABN);by Scholarship Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica de la Universidad Nacional Autónoma de México(PAPIIT-UNAM)IA203319 and PAPIIT-UNAM IN216221 to(LERL)。

摘　　要：Norepinephrine plays an important role in motor functional recovery after a brain injury caused by ferrous chloride.Inhibition of norepinephrine release by clonidine is correlated with motor deficits after motor cortex injury.The aim of this study was to analyze the role ofα-adrenergic receptors in the restoration of motor deficits in recovering rats after brain damage.The rats were randomly assigned to the sham and injury groups and then treated with the following pharmacological agents at 3 hours before and 8 hours,3 days,and 20 days after ferrous chloride-induced cortical injury:saline,clonidine,efaroxan(a selective antagonist ofα-adrenergic receptors)and clonidine+efaroxan.The sensorimotor score,the immunohistochemical staining forα-adrenergic receptors,and norepinephrine levels were evaluated.Eight hours post-injury,the sensorimotor score and norepinephrine levels in the locus coeruleus of the injured rats decreased,and these effects were maintained 3 days post-injury.However,20 days later,clonidine administration diminished norepinephrine levels in the pons compared with the sham group.This effect was accompanied by sensorimotor deficits.These effects were blocked by efaroxan.In conclusion,an increase inα-adrenergic receptor levels was observed after injury.Clonidine restores motor deficits in rats recovering from cortical injury,an effect that was prevented by efaroxan.The underlying mechanisms involve the stimulation of hypersensitiveα-adrenergic receptors and inhibition of norepinephrine activity in the locus coeruleus.The results of this study suggest thatαreceptor agonists might restore deficits or impede rehabilitation in patients with brain injury,and therefore pharmacological therapies need to be prescribed cautiously to these patients.

关 键 词：alpha2-adrenoceptors ambulatory behavior CLONIDINE cortical injury EFAROXAN functional recovery immunohistochemistry motor deficit norepinephrine sensorimotor score 

分 类 号：R285.5[医药卫生—中药学]