马铃薯StNPR4基因的克隆与功能分析  

Cloning and Functional Analysis of St NPR4 gene in Solanum tuberosum

作　　者：李登高[1] 林睿[1] 穆青慧 周娜 张焱如[1] 白薇[1] LI Denggao;LIN Rui;MU Qinghui;ZHOU Na;ZHANG Yanru;BAI Wei(Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《植物研究》2022年第5期821-829,共9页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(32060613)。

摘　　要：为探究StNPR4基因在马铃薯(Solanum tuberosum)中应对生物胁迫和非生物胁迫的功能,本研究通过克隆StNPR4的CDS序列和启动子序列,进行生物信息学分析;利用qRT-PCR进行组织表达特异性分析;同时构建了由其自身启动子驱动的StNPR4双元表达载体,转化马铃薯获得了转基因马铃薯,研究转基因马铃薯对水杨酸、致病疫霉和高盐胁迫的响应。结果显示:StNPR4具有典型的NPR1家族的功能结构域,启动子上具有响应于生物胁迫和非生物胁迫的顺式作用元件。StNPR4在叶中的表达量最高;StNPR4受SA诱导表达,且在转基因植株中的诱导表达程度高于对照;转基因马铃薯增强了对致病疫霉的抗性,在高盐胁迫下生根率更高。说明StNPR4不仅在马铃薯生物胁迫中发挥重要作用,而且在非生物胁迫中也扮演着重要角色。In order to clarify the function of St NPR4 in potato,the CDS and promoter sequence of St NPR4 were cloned and bioinformatics analysis was conducted.Tissue expression pattern was performed by q RT-PCR,and a binary expression vector of St NPR4 driven by its own promoter was constructed and transformed intopotato.Transgenic plants were used to examine the responses to salicylic acid,Phytophthora infestans and highsalt stress respectively.The results showed that St NPR4 had typical functional domains in NPR1 family with cisacting elements on the promoter in response to biotic and abiotic stresses.The St NPR4 expression level washighest in leaves St NPR4 was inductively expressed by SA and the induced expression was higher in transgenicplants than in the control.St NPR4 transgenic potato plants showed enhanced resistance to Ph.infestans andhigher rooting rate under high salt stress.This indicated that St NPR4 played an important role not only in bioticstress but also in abiotic stress of potato.

关 键 词：StNPR4 克隆 遗传转化 致病疫霉 盐胁迫 马铃薯 

分 类 号：Q939.92[生物学—微生物学]