HLA-B27阳性与阴性葡萄膜炎患者外周血中炎症因子表达研究  

Expression of inflammatory factors in peripheral blood of patients with HLA-B27 positive and negative uveitis

作　　者：王洪亚 郑文 戴永刚 周玮琰 孙进学 Hong-Ya Wang;Wen Zheng;Yong-Gang Dai;Wei-Yan Zhou;Jin-Xue Sun(Department of Clinical Laboratory,Shandong Provincial Third Hospital,Jinan 250031,Shandong Province,China;Department of Ophthalmology,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,Shandong Province,China)

机构地区：[1]山东省立第三医院检验科,山东省济南市250031 [2]山东第一医科大学附属省立医院眼科,山东省济南市250021

出　　处：《国际眼科杂志》2022年第9期1559-1563,共5页International Eye Science

基　　金：山东省医药卫生科技发展计划项目(No.202003041242)。

摘　　要：目的:通过分析比较人白细胞抗原-B27(HLA-B27)阳性及阴性葡萄膜炎患者外周血中炎症因子的表达变化情况,探讨其相关性。方法:回顾性研究。选取2020-01/2022-04我院初诊为葡萄膜炎的患者76例,采集静脉血分离有核细胞,用流式细胞术(直接免疫荧光法)检测HLA-B27,分成HLA-B27阳性组(≥90%)35例和HLA-B27阴性组(≤5%)41例。提取全血RNA,用RT-qPCR方法检测并比较白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、蛋白酶激活受体2(PAR2)、肿瘤坏死因子-α(TNF-α)、白细胞介素增强因子-3(ILF3)mRNA表达。结果:两组患者IL-1β、IL-10、PAR2、TNF-αmRNA表达均无差异(均P>0.05)。HLA-B27阳性组患者IL-6 mRNA较HLA-B27阴性组升高,ILF3 mRNA较HLA-B27阴性组降低(均P<0.05)。结论:HLA-B27阳性相关的葡萄膜炎患者外周血中炎性指标IL-6表达显著升高,ILF3较HLA-B27阴性组表达下降,可作为其辅助诊疗指标。·AIM:To explore the correlation by analyzing and comparing the expression of inflammatory factors in peripheral blood of human leukocyte antigen-B27(HLA-B27)positive and negative uveitis patients.·METHODS:All 76 patients first diagnosed with uveitis in our hospital from January 2020 to April 2022 were screened in this retrospective study.Nucleated cells were isolated from human venous blood,and HLA-B27 was detected by flow cytometry(direct immunofluorescence),the patients were divided into the HLA-B27-positive group(≥90%)in 35 cases and HLA-B27-negative group(≤5%)in 41 cases.The whole blood RNA was extracted.The mRNA expression of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),protease activated receptor 2(PAR2),tumor necrosis factor-α(TNF-α),interleukin enhancer-binding factor-3(ILF3)were detected and compared by RT-qPCR.·RESULTS:The IL-1β,IL-10,PAR2 and TNF-αmRNA were observed no difference between the two groups of patients(all P>0.05).The IL-6 mRNA in the patients of HLA-B27-positive group was higher than in the HLA-B27-negative group,the ILF3 mRNA was lower than that in the negative group(all P<0.05).·CONCLUSION:The expression level of IL-6 in peripheral blood was significantly increased and ILF3 was decreased in HLA-B27-positive group,which can be used as auxiliary indicators for diagnosis and treatment of HLA-B27-positive uveitis.

关 键 词：葡萄膜炎 人白细胞抗原-27(HLA-B27) 炎症因子 

分 类 号：R773.9[医药卫生—眼科]