谷氨酸棒杆菌细胞工厂构建与应用的研究进展  

Research progress on construction and application of Corynebacterium glutamicum cell factory

作　　者：上官玲玲 卢慧芳 夏会丽 陈雄 代俊[1,2,3,4] SHANGGUAN Lingling;LU Huifang;XIA Huili;CHEN Xiong;DAI Jun(School of Bioengineering and Food science,Hubei University of Technology,Wuhan 430068,China;Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei University of Technology,Wuhan 430068,China;Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology,Wuhan 430068,China;Hubei Collaborative Innovation Center of Industrial Fermentation,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院,湖北武汉430068 [2]发酵工程教育部重点实验室(湖北工业大学),湖北武汉430068 [3]工业发酵湖北省协同创新中心(湖北工业大学),湖北武汉430068 [4]工业微生物湖北省重点实验室(湖北工业大学),湖北武汉430068

出　　处：《食品与发酵工业》2022年第17期313-320,共8页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31871789,41876114);湖北省科技攻关计划项目(2020BGC010)。

摘　　要：谷氨酸棒杆菌作为工业生产氨基酸的一种核心菌株,已被开发成为能利用可再生原料生产多种有价值的天然或非天然化学品的多功能微生物细胞工厂。该文针对近年来谷氨酸棒杆菌细胞工厂的构建新技术与应用领域,首先综述了构建谷氨酸棒杆菌细胞工厂的新型诱变、高通量筛选及基因编辑技术,其次介绍了工程改造后的微生物细胞工厂高效合成有机酸、生物燃料和萜类化合物的应用,最后列举了高效的微生物细胞工厂利用可再生原料生产高附加值产品的经典实例,以期为扩展谷氨酸棒杆菌产物谱和底物谱的研究提供参考。Corynebacterium glutamicum, as a core strain for industrial production of amino acids, has been developed into a multifunctional microbial cell factory that can use renewable raw materials to produce a variety of valuable natural or unnatural chemicals. Aiming at the new construction technology and application fields of C. glutamicum cell factory in recent years, this review first summarizes the new mutation, high-throughput screening and gene editing technology for the construction of C. glutamicum cell factory, and then introduces the application of the engineered microbial cell factory in the efficient synthesis of organic acids, biofuels and terpenes. Finally, some classic examples of efficient microbial cell factory using renewable raw materials to produce high value-added products are listed. In summary, it provides a reference for expanding the scope of C. glutamicum product and substrate.

关 键 词：谷氨酸棒杆菌 细胞工厂 构建技术 高附加值产品 可再生资源 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]