多糖和激素对珠芽魔芋多叶形成的调控作用  

Regulation of polysaccharides and hormones on formation of multiple leaves in Amorphophallus bulbifer

作　　者：谭长龙 李思雅琦 钱澄 王昊[1] 年洪娟[1] 李昆志[1] TAN Changlong;LI Siyaqi;QIAN Cheng;WANG Hao;NIAN Hongjuan;LI Kunzhi(College of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《西北农林科技大学学报（自然科学版）》2022年第9期69-79,共11页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31960071,31760349)。

摘　　要：【目的】探究多糖和激素对珠芽魔芋多叶形成的作用,为调控珠芽魔芋多叶萌发生长提供理论依据。【方法】2020年,分别采集自然生长珠芽魔芋1叶期至4叶期幼苗,以当年仅着生1片复叶的花魔芋为对照,测定各时期的叶柄长、球茎鲜质量和根长,以及各器官中还原性多糖、淀粉以及玉米素(ZT)、赤霉素(GA_(3))、生长素(IAA)和脱落酸(ABA)含量变化;2021年,分别以20μg/L ZT溶液和40μg/L IAA溶液喷施珠芽魔芋幼苗,以喷施清水的珠芽魔芋幼苗为对照,分别采集幼苗1叶期至4叶期最新萌发嫩叶,测定各时期新叶中ZT、GA_(3)、IAA和ABA含量。【结果】(1)2020年,随着生长期延长,珠芽魔芋幼苗各生长时期的叶柄长、球茎鲜质量和根长均呈增加趋势,但是均低于花魔芋。各时期珠芽魔芋幼苗各器官中还原性多糖、淀粉含量均随着生长期的延长而增加;新叶中的还原性多糖和淀粉含量比老叶低。随着生长期的延长,珠芽魔芋幼苗叶片和根的ZT含量均明显增加,各时期新叶ZT含量比老叶低;而叶柄和球茎的ZT含量明显降低。随着生长期的延长,珠芽魔芋幼苗各器官中GA_(3)、IAA和ABA含量逐渐上升,新叶GA_(3)、IAA和ABA含量总体比老叶高。随着生长期的延长,各时期花魔芋不同器官糖和激素含量变化幅度较小,且均低于珠芽魔芋。(2)2021年,与对照相比,喷施ZT后,珠芽魔芋幼苗嫩叶ZT含量明显增加,且明显促进了幼苗侧叶形成速度;喷施IAA明显增加了幼苗IAA、GA_(3)和ABA含量,显著抑制了珠芽魔芋幼苗侧叶形成。【结论】珠芽魔芋中较高含量糖分和ZT及较低含量GA_(3)、IAA和ABA有利于珠芽魔芋侧叶萌发和生长。【Objective】This study explored the effects of polysaccharides and hormones on formation of Amorphophallus bulbifer multi-leaves to provide basis for regulating germination and growth of multi-leaves of A.bulbifer.【Method】In 2020,the seedlings of natural A.bulbifer from 1 to 4 leaf stages were collected,taking Amorphophallus konjac with only one compound leaf as the control.The petiole length,corm fresh quality and root length at each stage,as well as the changes of reducing polysaccharide,starch,zeatin(ZT),gibberellin(GA_(3)),auxin(IAA)and abscisic acid(ABA)in various organs were measured.In 2021,20μg/L ZT solution and 40μg/L IAA solution were sprayed on A.bulbifer seedlings,and A.konjac seedlings sprayed with water were used as control.Newly germinated young leaves from 1 to 4 leaf stages were collected and contents of ZT,GA_(3),IAA and ABA in new leaves at each stage were measured.【Result】(1)In 2020,with the extension of growth period,the petiole length,corm fresh quality and root of A.bulbifer seedlings in each growth period showed an increasing trend,but they were lower than that of A.konjac.The contents of reducing polysaccharides and starch in organs of A.bulbifer seedlings at each stage increased with the extension of growth period,and the contents of reducing polysaccharides and starch in new leaves were lower than those in old leaves.With the extension of growth period,the ZT contents in leaves and roots of A.bulbifer seedlings increased significantly with lower values in new leaves than in old leaves at each stage.The contents of ZT in petiole and corm decreased significantly.With the extension of growth period,the contents of GA_(3),IAA and ABA in organs of seedlings gradually increased,and the contents of GA_(3),IAA and ABA in new leaves were higher than those in old leaves.With the extension of growth period,the changes in sugar and hormone contents in different organs of A.konjac at each stage decreased and they were lower than those of A.bulbifer.(2)In 2021,the content of ZT in young

关 键 词：珠芽魔芋 多叶形成 还原性多糖 淀粉 激素 

分 类 号：S632.3[农业科学—蔬菜学]