PEI-SPIO包裹的siRNA在小鼠肝癌存留时间的研究  

Study on the retention of PEI-SPIO coated siRNA in mouse orthotopic liver cancer model

作　　者：杨针 张文智[1] 卢鹏 YANG Zhen;ZHANG Wenzhi;LU Peng(Department of Hepatobiliary Surgery,Hainan Hospital of PLA General Hospital,Sanya,Hainan 572000,China)

机构地区：[1]解放军总医院海南医院肝胆外科,海南三亚572000

出　　处：《重庆医学》2022年第17期2887-2890,共4页Chongqing medicine

基　　金：海南省自然科学基金项目(817357);海南省三亚市医疗卫生科技创新项目(2016YW13)。

摘　　要：目的研究聚乙烯亚胺修饰的磁性纳米颗粒(PEI-SPIO)包裹的小干扰RNA(siRNA)在小鼠原位肝癌中的持续时间。方法利用稳定表达荧光素酶肝癌luc-hepa1-6细胞系在C57BL/6小鼠肝脏建立小鼠原位肝癌模型,在建立肝癌模型后第21天通过小动物活体成像观察小鼠肝脏组织中成瘤情况及肿瘤大小。在第21天尾静脉注射PEI-SPIO包裹的siRNA(Cy5荧光标记),分别在2、8、24 h通过LuminaⅡ活体成像监测siRNA在小鼠体内的分布情况。最后灌注取材取离体的肝癌组织制备石蜡切片进行普鲁士蓝染色观察肿瘤组织中的磁性纳米颗粒的情况。结果小动物活体成像显示小鼠原位肝癌建立,PEI-SPIO与Cy5-siRNA的复合物能够到达肿瘤并在肿瘤组织中持续聚集,并能明显延长siRNA在小鼠肿瘤中的滞留时间,肿瘤组织的普鲁士蓝染色证明PEI-SPIO包裹的siRNA能够进入小鼠原位肝癌中且能在肝癌中持续存在。结论PEI-SPIO包裹的siRNA能够到达肝癌并能延长其持续时间。Objective To investigate the retention of polyethyleneimine modified magnetic nanoparticles(PEI-SPIO)coated siRNA in mouse orthotopic liver cancer model.Methods The mouse orthotopic liver cancer model was established in C57BL/6 mouse liver by stably expressing(luciferase)luc-hepa1-6 cell line.The tumor formation and tumor size of the mouse were observed by vivo imaging of small animals on the 20th day after the establishment of the model.On the 21th day,PEI-SPIO-encapsulated siRNA(Cy5-fluorescent labelled)was injected into the tail vein,and the distribution of siRNA in mice was monitored by Lumina II in vivo at 2 h,8 h and 24 h respectively.Finally,the hepatic tissue of the isolated body was taken and the paraffin sections were prepared for Prussian blue staining to observe the magnetic nanoparticles in the tumor tissue.Results The vivo imaging of small animals showed that mouse orthotopic liver cancer was stably established,and the complex of PEI-SPIO and Cy5-siRNA could reach the tumor and continue to aggregate in the tumor tissue.Prussian blue staining of the tumor tissue proved that the polyethyleneimine modified magnetic nanoparticle package siRNA can enter mouse orthotopic liver cancer and persist in liver cancer.Conclusion PEI-SPIO encapsulated siRNA can aggregate in mouse orthotopic liver cancer and extend its duration.

关 键 词：聚乙烯亚胺 磁性纳米颗粒 肝细胞肝癌 小干扰RNA 动物模型 

分 类 号：R735.7[医药卫生—肿瘤]