EphB4抑制早产大鼠视网膜病变新生血管的实验研究  

Experimental study on the inhibition of EphB4 on neovascularization in retinopathy of prematurity

作　　者：李游 陈晓隆[2] 张玉强 LI You;CHEN Xiaolong;ZHANG Yuqiang(Jinzhou AIER Eye Hospital,AIER Eye Hospital Group,Jinzhou,Liaoning 121001,China;Department of Ophthalmology,Shengjing Hospital of China Medical University,Shenyang,Liaoning 110004,China;Institute of Orthopaedics,Jinzhou Medical University,Jinzhou,Liaoning 121001,China)Objec)

机构地区：[1]爱尔眼科医院集团锦州爱尔眼科医院,辽宁锦州121001 [2]中国医科大学附属盛京医院眼科,沈阳110004 [3]锦州医科大学骨科研究所,辽宁锦州121001

出　　处：《重庆医学》2022年第17期2896-2900,共5页Chongqing medicine

基　　金：辽宁医学院校长基金项目(XZJJ20140221)。

摘　　要：目的建立大鼠氧诱导视网膜病变模型模拟人类早产儿视网膜病变,研究促红细胞生成肝细胞激酶(Eph)B4/促红细胞生成肝细胞激酶受体相互作用蛋白(Ephrin)B2对视网膜新生血管形成的影响,以及EphB4对视网膜病变新生血管的抑制作用。方法将80只新生SD大鼠分成模型组、干预组、对照组和正常组,每组20只。模型组建立大鼠氧诱导视网膜病变疾病模型,干预组建立氧诱导视网膜病变模型并在玻璃体腔内注射EphB4,对照组建立大鼠氧诱导视网膜病变模型并于玻璃体腔内注射生理盐水。每组17 d后处死并摘取双眼眼球,制作视网膜铺片和视网膜冰冻切片,分别做视网膜血管的ADP酶染色和EphB4/EphrinB2免疫荧光染色。取视网膜组织进行实时荧光定量PCR分析EphB4 mRNA。结果模型组和对照组视网膜可观察到大量新生血管及视网膜出血,而干预组视网膜的新生血管数量及出血较模型组和对照组明显减少。免疫荧光结果显示模型组和对照组视网膜EphB4和EphrinB2蛋白呈高表达,干预组EphB4和EphrinB2表达较模型组和对照组明显减少。实时荧光定量PCR显示模型组和对照组大鼠视网膜EphB4 mRNA呈高表达,干预组EphB4 mRNA低于模型组和对照组(P<0.05)。结论视网膜新生血管的增殖过程中EphB4/EphrinB2表达过高,EphB4可有效抑制氧诱导视网膜病变新生血管的形成。Objective A rat model of oxygen-induced retinopathy was established,to simulate retinopathy of premature infants and study the effect of erythropoiesis hepatocyte kinase(Eph)B4/erythropoiesis hepatocyte kinase receptor protein(Ephrin)B2 on retinal neovascularization and the inhibitory effect of EphB4 on retinal neovascularization.Methods A total of 80 newborn SD rats were randomly divided into the model group,the intervention group,the normal group,and the control group,with 20 rats in each group.In the model group,the rat model of oxygen induced retinopathy was established.In the intervention group,the rat model of oxygen induced retinopathy was established and EphB4 was injected into vitreous cavity.In the control group,the rat model of oxygen induced retinopathy was established and the same amount of normal saline was injected into the vitreous cavity.After 17d,the rats were killed and their eyeballs were removed,retinal paving and frozen sections were made,the retinal vessels were stained with ADP enzyme staining,and the expression of EphB4/EphrinB2 protein in retina was observed with immunofluorescence.Retinal tissues were taken for real time-PCR to analyze the expression of EphB4 mRNA.Results A large number of neovascularization and retinal hemorrhage were observed in the model group and the control group,while the number of neovascularization and retinal hemorrhage in the intervention group were significantly reduced.Immunofluorescence showed that EphB4 and EphrinB2 were highly expressed in the model group and the control group,while the expression of EphB4 and EphrinB2 were significantly decreased in the intervention group.Real time-PCR showed that EphB4 mRNA was highly expressed in the retinal of rats in the model group and the control group,and EphB4 mRNA in the intervention group was lower than that in the model group and the control group(P<0.05).Conclusion The expression of EphB4/EphrinB2 was high in the proliferation of retinal neovascularization,and EphB4 can effectively inhibit the formation of re

关 键 词：氧诱导 早产儿视网膜病变 新生血管 促红细胞生成肝细胞激酶B4 促红细胞生成肝细胞激酶受体相互作用蛋白B2 

分 类 号：R779.7[医药卫生—眼科]