基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测  

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

作　　者：于绍楠[1,2] 任玲玲 任立群 白应洁[1] 刘桂锋 张婳[3] 王振新[3] YU Shao-Nan;REN Ling-Ling;REN Li-Qun;BAI Ying-Jie;LIU Gui-Feng;ZHANG Hua;WANG Zhen-Xin(Department of Radiology,China-Japan Union Hospital of Jilin University,Changchun 130033,China;School of Pharmaceutical Sciences,Jilin University,Changchun 130021,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]吉林大学中日联谊医院放射线科,长春130033 [2]吉林大学药学院,长春130021 [3]中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春130022

出　　处：《分析化学》2022年第9期1299-1307,共9页Chinese Journal of Analytical Chemistry

基　　金：吴阶平医学基金项目(Nos.320675019089-40,320675019089-38);国家自然科学基金项目(No.61901438)资助。

摘　　要：构建了一种基于上转换纳米粒子(Upconversion nanoparticles,UCNP)-金纳米棒(Gold nanorods,GNR)的荧光共振能量转移(Fluorescence resonance energy transfer,FRET)免疫分析方法用于灵敏检测癌胚抗原(Carcinoembryonic antigen,CEA)。以UCNP作为供体,GNR作为受体,将CEA抗体通过共价偶联的方式分别修饰在UCNP和GNR表面,形成抗体偶联物UCNP-cAb和GNR-dAb,当引入CEA后,通过抗原-抗体相互作用形成“三明治”夹心复合物,导致UCNP与GNR相互靠近发生FRET,并且荧光淬灭效率与CEA浓度呈正相关。基于此建立了检测CEA的FRET免疫分析方法,线性范围为0.01~100 ng/mL,检出限(S/N=3)为0.01 ng/mL。采用构建的FRET免疫分析方法实现了对缓冲溶液和人血清样本中的CEA的检测。本方法具有良好的选择性,有望用于临床检测,为相关癌症的早期诊断、治疗及预后监测等提供了新方法。A fluorescence resonance energy transfer(FRET)immunoassay based on upconversion nanoparticles(UCNP)and gold nanorods(GNR)was constructed for sensitive detection of carcinoembryonic antigen(CEA).UCNP was employed as the donor and GNR was employed as the acceptor.The CEA antibody was covalently attached on the surface of UCNP and GNR to form UCNP-cAb and GNR-dAb conjugates,respectively.After introduction of CEA,a“sandwich”complex was formed through the antigen-antibody specific interaction,which resulted in the close proximity of UCNP and GNR to generate FRET.And the fluorescence quenching efficiency was positively correlated with the concentration of CEA.The constructed FRET immunoassay realized the detection of CEA in both buffer solution and human serum samples with a limit of detection(S/N=3)of 0.01 ng/mL,a linear range from 0.01 ng/mL to 100 ng/mL and good selectivity.It was expected that this FRET immunoassay could be further used in clinical applications and provided new methods for early diagnosis,treatment and prognosis monitoring of related cancers.

关 键 词：上转换纳米粒子 金纳米棒 荧光共振能量转移 癌胚抗原 定量检测 

分 类 号：R730.43[医药卫生—肿瘤] O657.3[医药卫生—临床医学]