基于金标银染技术的冈田酸核酸适配体传感器的研究  

An Okadaic Acid Aptasensor Based on Silver-enhanced Gold Nanoparticle Label

作　　者：童萍[1,2,3] 张慧玲 陆伟 张文敏 张兰 TONG Ping;ZHANG Hui-Ling;LU Wei;ZHANG Wen-Min;ZHANG Lan(Fujian College Association Instrument Analysis Center,Fuzhou University,Fuzhou 350116,China;College of Environment and Safety Engineering,Fuzhou University,Fuzhou 350116,China;Key Laboratory for Analytical Science of Food Safety and Biology,Ministry of Education,College of Chemistry,Fuzhou University,Fuzhou 350116,China;School of Chemical and Biological Technology,Minjiang Teachers College,Fuzhou 350108,China)

机构地区：[1]福州大学福建省高校测试中心,福州350116 [2]福州大学环境与安全工程学院,福州350116 [3]福州大学化学学院,教育部食品安全与生物分析重点实验室,福州350116 [4]闽江师范高等专科学校化学与生物技术学院,福州350108

出　　处：《分析化学》2022年第9期1355-1363,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21605022);福建省科技重大专项专题项目(No.2020YZ019007);福州市科技计划项目(No.2019-S-66)资助。

摘　　要：利用纳米金(AuNPs)可在对苯二酚作为弱还原剂条件下特异性催化银离子沉积的金标银染信号放大特性,制备了一种高效、灵敏检测冈田酸(OA)的电化学核酸适配体传感器,实现了复杂样品中痕量OA的检测。当溶液中存在OA时,电极表面的OA适配体可与溶液中的OA特异性结合而从电极表面解离下来,标记了AuNPs的信号探针再通过与游离的捕获探针杂交反应将AuNPs修饰至电极表面,然后将电极浸泡到银染试剂中进行银染反应,最后通过方波伏安法(SWV)直接检测纳米银的氧化信号,从而实现对OA的检测。在最佳实验条件下,此传感器的线性范围为10.0 pg/mL~500.0 ng/mL,检出限(3σ/k)为8.8 pg/mL。将此传感器应用于淡菜样品中OA的检测,测得其可食用部分中OA的含量为36.0 ng/g,低于欧盟的限量标准(160.0 ng/g),加标回收率为94.2%~112.0%。Based on silver-enhanced gold nanoparticles(AuNPs)label technique that AuNPs could specifically catalyze the deposition of silver ions using hydroquin as a weak reducing agent,an efficient and sensitive electrochemical aptasensor was developed for detection of trace okadaic acid(OA).When the sample solution containing OA,the OA aptamer dissociated from the dsDNA on electrode surface due to the specific binding between OA and OA aptamer,AuNPs labeled signal probe(Sp)could hybridize with the resulted free capture probe(Cp)on the electrode surface.And then,the electrode was immersed in a silver staining reagent to perform following sliver staining.Finally,the oxidation signal of the nano-silver was directly detected by square wave voltammetry(SWV).Under the optimal experimental conditions,the linear range of the established OA aptasensor was 10.0 pg/mL-500.0 ng/mL,and the detection limit(3σ/k)was 8.8 pg/mL.The sensor was applied to detection of mussels.The experimental results showed that the content of OA in the edible part of mussels was 36.0 ng/g,which was lower than the permitted maximal limit of European Union(160.0 ng/g),and the recoveries were 94.2%-112.0%.

关 键 词：金标银染技术 冈田酸 核酸适配体传感器 信号放大 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.1[轻工技术与工程—食品科学与工程] TP212[理学—分析化学]