白耳黄鸡冠齿数性状全基因组关联分析  

Genome-wide Association Analysis on Comb Teeth Traits in White-ear Yellow Chicken

作　　者：张文武 邱峰芳[1] 刘影 许继国 ZHANG Wenwu;QIU Fengfang;LIU Ying;XU Jiguo(College of Chemical,Biology and Materials Science,East China University of Technology,Nanchang,Jiangxi 330013;Institute of Biological Technology,Nanchang Normal University/Jiangxi Province Key Lab of Genetic Improvement of Indigenous Chicken Breeds,Nanchang,Jiangxi 330029)

机构地区：[1]东华理工大学化学生物与材料科学学院,江西南昌330013 [2]南昌师范学院生物技术研究所,江西地方鸡种遗传改良重点实验室,江西南昌330029

出　　处：《中国家禽》2022年第8期16-20,共5页China Poultry

基　　金：国家自然科学基金项目(32160784);广东省农业动物基因组学与分子育种重点实验室开放课题;江西省自然科学基金项目(20171BAB204013)。

摘　　要：试验采用全基因组关联分析(Genome-wide association study,GWAS)寻找与白耳黄鸡冠齿数性状相关的候选基因。以82只白耳黄鸡为试验材料,采集血液提取DNA,然后用Illumina鸡60 K芯片进行基因型分型,利用PLINK 1.90对分型结果进行质控后,采用GEMMA软件对SNPs(Single nucleotide polymorphisins)与性状做GWAS分析,定位出显著位点。结果显示:与冠齿数相关的SNP位点有7个,位于白耳黄鸡1、2、4号染色体上。通过基因功能注释,预测WNT9A基因可作为白耳黄鸡冠齿数的重要候选基因。研究结果将为白耳黄鸡冠齿数的选择提供候选分子标记,为地方鸡标记辅助选择提供新的思路。The objective of this experiment was to find candidate genes related to the comb teeth in White-ear Yellow chicken by genome-wide association analysis(GWAS). Using 82 White-ear Yellow chicken as test materials, the genotype was got by Illumina 60 K SNP chip after genomic DNA extracted from blood samples. After quality control of the typing results with PLINK 1.90, GEMMA software was used to perform GWAS analysis on SNPs and traits, and significant sites were located. The results showed that a total of 7 significant SNPs with the comb teeth in White-ear Yellow chicken, which located at chromosomes of No. 1, 2, 4. Through gene function annotation, WNT9A was predicted to be an important candidate gene for the comb teeth of White-ear Yellow chicken. These results would provide candidate molecular markers for White-ear Yellow chicken breeding and provide new ideas for marker-assisted selection in local chickens.

关 键 词：全基因组关联分析 白耳黄鸡 单核苷酸多态性 冠齿数 

分 类 号：S831.2[农业科学—畜牧学]