基于生物信息学分析微小染色体维持蛋白2在头颈部鳞状细胞癌中的表达及意义  

Expression and significance of minichromosome maintenance protein 2 in head and neck squamous cell carcinoma were analyzed based on bioinformatics

作　　者：黄舒婷 温炬[2] 江奕恒[1] 秦思[2] HUANG Shuting;WEN Ju;JIANG Yiheng;QIN Si(Department of Outpatient,Civil Aviation Hospital,Guangdong Second Provincial General Hospital,Guangdong Province,Guangzhou510405,China;Department of Dermatology and Venereology,Guangdong Second Provincial General Hospital,Guangdong Province,Guangzhou510317,China)

机构地区：[1]广东省第二人民医院民航院区门诊部,广东广州510405 [2]广东省第二人民医院皮肤性病科,广东广州510317

出　　处：《中国当代医药》2022年第25期5-8,12,共5页China Modern Medicine

基　　金：广东省第二人民医院青年科研项目(YQ2019-002)。

摘　　要：目的探讨头颈部鳞状细胞癌(HNSCC)中微小染色体维持蛋白2(MCM2)的表达情况及意义。方法通过基因表达公共(GEO)数据库比较MCM2在HNSCC和正常组织中的表达差异。利用UALCAN数据库分析不同因素下MCM2在HNSCC和正常组织中的表达情况;TIMER数据库分析MCM2与免疫细胞的相关性;运用Linked Omics数据库和STRING数据库研究与MCM2密切相关的基因。最后运用GSCALite数据库分析相关关键基因的药物靶点。结果MCM2在HNSCC中表达高于正常组织,差异有统计学意义(P<0.05)。MCM2在不同年龄段、性别、肿瘤分级、临床分期和淋巴转移的HNSCC中表达均高于正常组织,且人乳头瘤病毒(HPV)阳性患者MCM2表达高于HPV阴性患者,差异有统计学意义(P<0.05)。HNSCC中MCM2的表达与免疫细胞的浸润水平成正相关(P<0.05)。GSCALite数据库分析显示MCM2受NPK76-II-72-1、Navitoclax、GSK1070916和I-BET-762等药物调节。结论MCM2在HNSCC中高表达,可作为HNSCC诊断和药物治疗的新靶点。Objective To investigate the expression and significance of minichromosome maintenance protein 2(MCM2)in head and neck squamous cell carcinoma(HNSCC).Methods The Gene Expression Omnibus(GEO)databases were adopted to compare the expression of MCM2 in HNSCC with normal tissues,and the UALCAN database was used to analyze the expression of MCM2 in HNSCC and normal tissues in different conditions.The association of MCM2 with immune cells was analyzed by the TIMER database.Linked Omics and STRING databases were used to study genes closely related to MCM2.Finally,GSCALite database was used to analyze drug targets of the related key genes.Results The expression of MCM2 in HNSCC was higher than that in normal tissues,the difference was statistically significant(P<0.05).The expressions of MCM2 in HNSCC of different age groups,gender,tumor grades,clinical stages and lymphatic metastasis were higher than that in normal tissues,the expression of MCM2 in human papilloma virus(HPV)positive patients was higher than that in HPV negative patients,the difference was statistically significant(P<0.05).The expression of MCM2 in HNSCC was positively correlated with the infiltration level of immune cells(P<0.05).GSCALite database analysis showed MCM2 was regulated by nPK76-II-72-1,Navitoclax,GSK1070916 and I-BET-762.Conclusion MCM2 was highly expressed in HNSCC,which could be used as a new target for diagnosis and drug treatment of HNSCC.

关 键 词：头颈部鳞状细胞癌 微小染色体维持蛋白2 免疫浸润 治疗靶点 

分 类 号：R739.91[医药卫生—肿瘤]