基于TCGA数据库探索KDM5c通过影响RNA m6A修饰调控结肠癌预后机制  

Exploring the mechanism of KDM5c regulating colon cancer prognosis by affecting RNA m6A modification based on TCGA database

作　　者：林海珊[1] 尚昆[1] 曹邦伟[1] LIN Hai-shan;SHANG Kun;CAO Bang-wei(Cancer Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院肿瘤中心,北京100050

出　　处：《临床和实验医学杂志》2022年第15期1585-1588,共4页Journal of Clinical and Experimental Medicine

基　　金：北京市自然科学基金青年项目(编号:7214220);国家自然科学基金青年项目(编号:82100539);首都医科大学附属北京友谊医院种子计划(编号:YYZZ202034);国家自然科学基金面上项目(编号:82173056)。

摘　　要：目的 通过对癌症基因组图谱(TCGA)数据库中结肠腺癌(COAD)转录组数据进行分析,区别KDM5c高低表达组结肠癌预后区别及两组差异基因分析探索可能影响预后的机制。方法 从TCGA数据库中下载COAD的转录组数据,以KDM5c mRNA水平中位数界,分为KDM5c高低表达组,进行生产分析,利用R语言edge R包筛选出差异基因后,对差异基因进行GESA分析及RNA m6A相关蛋白分析。结果 与癌旁组织相比,KDM5c高表达于结肠腺癌组织中,且Logistics回归分析发现KDM5c高表达结肠腺癌好发与KDM5c在多结肠腺瘤转化所致结肠癌、女性肠癌、腺癌类型肠癌以及右半结肠癌,KDM5c影响结肠癌5年生存率。同时KDM5c差异表达基因上调多种促癌基因,且KDM5c差异表达影响11种RNA m6A蛋白表达,其中可能通过影响METTL14基因CpG岛甲基化下调其表达。结论 KDM5c可能通过影响RNA m6A相关蛋白表达影响结肠腺癌预后。Objective To analyze the transcriptome data of colon adenocarcinoma(COAD)in The Cancer Genome Atlas(TCGA)database,differentiate the prognosis of colon cancer between high and low KDM5c expression groups,and analyze the differential genes between the two groups to explore the mechanism that may affect the prognosis.Methods The transcriptome data of COAD was downloaded from the TCGA database,and divided into KDM5c high and low expression groups based on the median boundary of KDM5c mRNA level.Differential genes were analyzed using GESA grading,and RNA m6A-related proteins were analyzed.Results Compared with adjacent tissues,KDM5c was highly expressed in colon adenocarcinoma tissues,and Logistic regression analysis found that the high expression of KDM5c in colon adenocarcinoma was associated with the type of colon cancer,female bowel cancer and adenocarcinoma caused by the transformation of KDM5c in multiple colon adenomas.Colon cancer and right colon cancer,KDM5c affects colon cancer 5-year survival.At the same time,the differentially expressed genes of KDM5c up-regulated a variety of tumor-promoting genes,and the differential expression of KDM5c affected the expression of 11 RNA m6A proteins,which may be down-regulated by affecting the CpG island methylation of the METTL14 gene.Conclusion KDM5c may affect the prognosis of colon adenocarcinoma by affecting the expression of RNA m6A-related protein.

关 键 词：结肠腺癌 RNA m6A修饰 预后 

分 类 号：R735.35[医药卫生—肿瘤]