肺癌组织中TLR3、RKIP蛋白表达及与肿瘤增殖、侵袭活性的相关关系  

Expression of TLR3 and RKIP proteins in lung cancer and their relationship with cell proliferation and invasion abilities

作　　者：胡海舰 隋倩 曹永清 HU Hai-jian;SUI Qian;CAO Yong-qing(Department of Hematology and Oncology,Changsha First Hospital,Changsha Hunan 410005,China)

机构地区：[1]长沙市第一医院血液肿瘤科,湖南长沙410005

出　　处：《临床和实验医学杂志》2022年第16期1693-1696,共4页Journal of Clinical and Experimental Medicine

基　　金：湖南省自然科学基金(编号:2018JJ3365)。

摘　　要：目的研究肺癌组织中Toll样受体3(TLR3)、Raf激酶抑制蛋白(RKIP)蛋白表达及与肿瘤增殖、侵袭活性的相关关系。方法回顾性选取2019年8月至2022年2月长沙市第一医院收治的94例肺癌患者作为研究对象,对比肺癌组织和癌旁正常组织TLR3、RKIP蛋白表达情况,同时对比阴性和阳性TLR3、RKIP蛋白表达肿瘤增殖相关蛋白(P53、NF2、Nanog)、侵袭活性相关蛋白[组蛋白去乙酰化酶1(HDAC1)、牛磺酸上调基因1(TUG1)、Rab11],并分析TLR3、RKIP蛋白表达与P53、NF2、Nanog、HDAC1、TUG1以及Rab11水平的相关性。结果肺癌组织患者TLR3和RKIP阴性率为91.48%、90.42%,均显著高于癌旁正常组织(11.70%、12.76%),差异均有统计学意义(P<0.05)。TLR3和RKIP阴性表达患者肿瘤增殖相关蛋白P53、NF3表达均显著低于TLR3和RKIP阳性表达患者,肿瘤增殖相关蛋白Nanog以及侵袭活性相关蛋白HDAC1、TUG1、Rab11表达均显著高于TLR3和RKIP阳性表达患者,差异均有统计学意义(P<0.05)。经过Pearson相关性分析发现,TLR3、RKIP蛋白表达与肿瘤增殖相关蛋白P53、NF3呈正相关(P<0.01),与肿瘤增殖相关蛋白Nanog以及侵袭活性相关蛋白HDAC1、TUG1、Rab11呈负相关(P<0.01)。结论肺癌组织中TLR3、RKIP蛋白呈低表达水平,且与肿瘤增殖和侵袭活性呈明显的相关性。Objective To investigate the expression of toll-like receptor 3(TLR3)and Raf kinase inhibitor protein(RKIP)proteins in lung cancer tissues,and to discuss their relationship with cell proliferation and invasion abilities.Methods Ninety-four patients with lung cancer in Changsha First Hospital from August 2019 to February 2022 were enrolled.Expression levels of TLR3 and RKIP proteins in lung cancer tissues and adjacent normal tissues were detected.The expression levels of cell proliferation-associated proteins(P53,NF2 and Nanog)and invasive-associated proteins[histone deacetylase 1(HDAC1),taurine up-regulated gene 1(TUG1),Rab11]were compared between patients with negative and positive expression of TLR3 and RKIP.Thereafter,the correlation of TLR3 and RKIP protein expressions with P53,NF2,Nanog,HDAC1,TUG1 and Rab11 levels was analyzed.Results The negative expression rates of TLR3 and RKIP proteins were 91.48%and 90.42%in lung cancer tissues,which were obviously higher than those of adjacent normal tissues(11.70%,12.76%),the differences were statistically significant(P<0.05).The expression of cell proliferation-associated proteins P53 and NF3 in patients with negative expression of TLR3 and RKIP were remarkably lower than those in patients with positive expression of TLR3 and RKIP,moreover,the expression of tumor proliferation-associated proteins Nanog and invasion-associated proteins HDAC1,TUG1 and Rab11 were significantly higher than those in patients with positive expression of TLR3 and RKIP,the differences were statistically significant(P<0.05).Pearson correlation analysis showed that the expression of TLR3 and RKIP proteins was positively correlated with tumor proliferation-related proteins P53 and NF3(P<0.01),and negatively correlated with tumor proliferation-related proteins Nanog and invasion activity-related proteins HDAC1,TUG1 and Rab11(P<0.01).Conclusion Patients with lung cancer have down-regulated expression of TLR3 and RKIP proteins,and the expression are significantly correlated with tumor cell prolife

关 键 词：肺癌组织 TOLL样受体3 肿瘤增殖 侵袭活性 相关性 

分 类 号：R734.2[医药卫生—肿瘤]