宫颈癌组织中HPV16 E6、E7蛋白水平与患者临床病理特征及预后的关系  

The relationship of HPV16 E6,E7 protein levels in cervical cancer tissues with clinical characteristics and prognosis

作　　者：路玲 张媛媛[2] 徐瑶[1] 吴宝华 徐蕾[1] 吴海燕[1] LU Ling;ZHANG Yuan-yuan;XU Yao;WU Bao-hua;XU Lei;WU Hai-yan(Department of Gynecology,the First Affiliated Hospital of Chengdu Medical College,Sichuan Province,Chengdu 610500,China;Department of Gynecology,the Affiliated Cancer Hospital of Xinjiang Medical University,Xinjiang Province,Urumqi 830054,China)

机构地区：[1]成都医学院第一附属医院妇科,四川成都610500 [2]新疆医科大学附属肿瘤医院妇科,新疆乌鲁木齐830054

出　　处：《河北医科大学学报》2022年第8期931-934,955,共5页Journal of Hebei Medical University

基　　金：高层次人才科研启动基金项目(CYFY-GQ33)。

摘　　要：目的探究宫颈癌组织中16型人乳头瘤病毒E6蛋白(human papilloma virus type 16 E6 protein,HPV16 E6)、16型人乳头瘤病毒E7蛋白(human papilloma virus type 16 E6 protein,HPV16 E7)水平与患者临床病理特征及预后的关系。方法选取行宫颈癌根治术87例患者的肿瘤组织、癌旁组织、正常宫颈组织标本进行研究,采用免疫组织化学法检测组织中HPV16 E6、HPV16 E7蛋白表达情况,并结合患者临床病理特征进行分析,采用Kaplan-Meier生存曲线比较患者3年生存情况,采用COX回归分析各项因素对患者预后的影响。结果免疫组化结果显示肿瘤组织中HPV16 E6、HPV16 E7阳性率高于癌旁组织和正常组织(P<0.05);HPV16 E6及HPV16 E7蛋白阳性表达患者3年生存率低于阴性表达患者(P<0.05);多因素分析显示HPV16 E6、HPV16 E7阳性是宫颈癌患者预后不良的独立危险因素(P<0.05)。结论HPV16 E6、HPV16 E7蛋白在宫颈癌组织中阳性表达,与患者临床病理特征和预后相关,可能作为治疗宫颈癌的新靶点发挥作用。Objective To explore the relationship of the levels of human papilloma virus E6 protein(HPV16 E6)and human papilloma virus E7 protein(HPV16 E7)in cervical cancer tissues with the clinical characteristics and prognosis of patients.Methods The tumor tissue,adjacent tissue,and normal cervical tissue specimens of 87 patients undergoing radical cervical cancer surgery were selected for research.Immunohistochemistry was used to detect the expression of HPV16 E6 and HPV16 E7 proteins in the tissues for analysis by combining the clinicopathological characteristics of the patients.Kaplan-Meier survival curve was used to compare the 3-year survival of the patients,and the COX regression analysis was used to analyze the effect of various factors on the prognosis of the patients.Results Immunohistochemical results showed that the positive rates of HPV16 E6 and HPV16 E7 in tumor tissues were higher than those in adjacent tissues and normal tissues(P<0.05),and the 3-year survival rate of patients with positive expression of HPV16 E6 and HPV16 E7 protein was lower than that of patients with negative expression(P<0.05).Multivariate analysis showed that HPV16 E6 and HPV16 E7 were independent risk factors for poor prognosis in patients with cervical cancer(P<0.05).Conclusion HPV16 E6 and HPV16 E7 proteins are positively expressed in cervical cancer tissues,which are related to the clinicopathological characteristics and prognosis of patients,and may play a role as new targets for the treatment of cervical cancer.

关 键 词：宫颈肿瘤 16型人乳头瘤病毒E6蛋白 16型人乳头瘤病毒E7蛋白 

分 类 号：R737.33[医药卫生—肿瘤]