DNA脱碱基位点的检测方法及其生物学研究进展  

Research on Detection Methods and Biology of DNA Abasic Sites

作　　者：武海江 张雅姣 袁舒妍 徐华 谢剑炜 WU Hai-jiang;ZHANG Ya-jiao;YUAN Shu-yan;XU Hua;XIE Jian-wei(State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;Wuya College of Innovation,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]军事医学研究院毒物药物研究所,抗毒药物与毒理学国家重点实验室,北京100850 [2]沈阳药科大学无涯创新学院,辽宁沈阳110016

出　　处：《分析测试学报》2022年第9期1365-1374,共10页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(22104153)。

摘　　要：DNA中糖苷键断裂形成的脱碱基位点(脱嘌呤/嘧啶位点,AP位点)是常见的DNA损伤类型之一,由核苷酸自发水解产生,也是DNA碱基切除修复途径中的关键中间体。若修复不及时,可能会导致DNA复制阻滞和DNA链断裂,产生突变和细胞毒性,同时还会引起形成DNA交联或DNA-蛋白质交联的损伤,因此对于AP损伤的检测有助于理解细胞氧化应激损伤和基因毒性物质的毒性评价。目前检测AP位点的方法有14C或32P后标记法、酶联免疫吸附分析(ELISA)法和液相色谱-质谱(LC-MS)技术等。该文重点概述DNA中AP位点的检测方法和生物学研究进展,并展望了AP位点损伤相关研究的前景。The abasic sites(apurinic/apyrimidinic sites,AP sites)generated from the cleavage of Nglycosylic bond in DNA are one of the common lesions in DNA,which are formed via spontaneous hydrolysis,as well as a major intermediate during the base excision repair.If AP sites are not repaired timely,they may cause DNA replication stalling and DNA strand breaks,resulting in mutagenesis and cytotoxicity,and further cause DNA crosslinks or DNA-protein crosslinks.Therefore,analysis of AP sites will be helpful for the assessment of cellular oxidative stress damage or the toxicity evaluation of genotoxic chemicals.A variety of analytical methods have been developed for the detection of AP sites in DNA,including 14C or 32P postlabeling,enzyme-linked immunosorbent assay(ELISA)and liquid chromatography-mass spectrometry(LC-MS).The research progress in the detection methods and biology of AP sites in DNA is reviewed in this paper,and the research prospects of AP sites are presented.

关 键 词：脱碱基位点 DNA损伤 质谱 DNA链间交联 DNA-蛋白质交联 

分 类 号：O65[理学—分析化学] G353.11[理学—化学]