旋毛虫感染对高脂饮食肥胖小鼠的干预作用  

Intervention Effect of Trichinella spiralis Infection on High Fat Diet Induced Obesity in Mice

作　　者：仝明薇 李承瑶 康杰[3] 杨勇 TONG Mingwei;LI Chengyao;KANG Jie;YANG Yong(School of Basic Medical Science,Shanxi Medical University,Taiyuan 030000,China;Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,China;Science and Technology Center of Fenyang College,Shanxi Medical University,Fenyang 032200,China)

机构地区：[1]山西医科大学基础医学院,太原030000 [2]吉林大学动物医学院人兽共患病研究所,长春130062 [3]山西医科大学汾阳学院科技中心,汾阳032200

出　　处：《中国动物传染病学报》2022年第4期182-189,共8页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(32100742);山西省青年科技研究基金(201901D211318);山西省高等学校科技创新项目(2019L0436)。

摘　　要：流行病学分析显示,蠕虫感染率和肥胖等代谢疾病呈负相关,然而其作用机制未被完全阐明。本文探究了旋毛虫(Trichinella spiralis)感染对高脂饮食(HFD)肥胖小鼠的干预作用,并初步分析其作用机制。以高脂饮食构建肥胖模型,分为3组:ND组给予基础饲料,其他两组高脂饲喂。4周后,HFD+inf组口服感染250条肌肉虫期旋毛虫,各组继续饲喂8周。每周称重,实验末期统计肝脏和附睾脂肪湿重;检测血清总胆固醇(TC)和甘油三酯(TG)含量;HE染色观察附睾脂肪组织病理学形态;油红O染色观察肝脏组织内脂滴情况;荧光定量PCR方法分析附睾脂肪中脂代谢基因表达。结果显示,与HFD组比较,HFD+inf组体重增加明显降低,血清中TC和TG的升高程度有所降低;HE显示附睾脂肪细胞的体积增大得到改善;同时,小鼠肝脏质量增加呈现降低趋势,油红O染色可见肝脏脂质变性得以纠正;此外,旋毛虫感染还可下调附睾脂肪组织中脂质合成相关基因并且上调脂质分解相关基因的mRNA表达水平。结果表明,旋毛虫可以通过减少机体脂质累积和加速脂质分解来影响脂质代谢,从而有效干预高脂诱导的肥胖,因此旋毛虫及其抗原可能为防治肥胖提供了一种新的策略。Epidemiological studies have indicated an inverse correlation between the prevalence of metabolic syndrome such as obesity and helminth infection although precise mechanism by which helminths intervene obesity and metabolic syndrome is still unknown.In this study,we attempted to explore the intervention effect and mechanism of Trichinella spiralis infection on high fat diet(HFD)induced obesity in mice.Mice were divided into three groups,among which one group of mice were fed with normal diet(ND)and other two groups with HFD.Four weeks later,one group of HFD mice(HFD+inf)were orally infected with 250 muscle larvae.After eight weeks,the body weight,liver and epididymal fat mass were tested for all 3 groups.Total cholesterol(TC)and triglyceride(TG)concentration were measured for serum samples and the histopathological changes of epididymal fats and livers were examined by HE or oil-red staining. Moreover, the mRNA expression levels of genes related to lipid metabolism were tested by qRT-PCR method. The results showed that the body weight gain and epididymal fat index of HFD+inf group were signifi cantly decreased and TC and TG concentrations in serum samples downward trended as compared to HFD group. The lipid accumulation in epididymal fats was suppressed as visualized in HE staining. Besides, the liver weight gains also declined and lipid degeneration of liver tissues was improved as observed by oil-red staining in HFD+inf group. Furthermore, T. spiralis infection downregulated the mRNA expression of genes related to lipid synthesis, such as FAS and PPAR-γ but upregulated mRNA expression levels of LPL, HSL and PPAR-α that were responsible of lipidolysis. In summary, our results indicated that T. spiralis infection improved the HFD induced obesity in mice, possibly through reducing lipid synthesis and accelerating lipidolysis, which might be used as a potential approach to antagonism of obesity.

关 键 词：旋毛虫 高脂饮食 肥胖 脂质代谢 

分 类 号：R589.2[医药卫生—内分泌] R532.14[医药卫生—内科学]