四唑染色法快速测定北乌头种子生活力  

Rapid determination of seed viability of Aconitum kusnezoffii by tetrazolium staining method

作　　者：隋昕 于营[1] 鲁海坤 于芸泽 郭靖[1] 姜业成 SUI Xin;YU Ying;LU Hai-kun;YU Yun-ze;GUO Jing;JIANG Ye-cheng(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China;Institute of Resources and Environment,Jilin Academy of Agricultural Sciences,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林省农业科学院资源与环境研究所,长春130112

出　　处：《湖北农业科学》2022年第15期150-152,共3页Hubei Agricultural Sciences

基　　金：吉林省科技发展计划项目(20180201034YY);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ISAPS);吉林省中药材种子种苗检测实验室项目(2018-S-44)。

摘　　要：为实现北乌头(Aconitum kusnezoffii)种子活力快速测定,通过4因素3水平正交试验,探究了四唑染色法(TTC)测定北乌头种子活力中各因素对染色效果的影响,并确定了最适染色条件。结果表明,在各因素对种子染色效果的影响中,染色时间>TTC浓度>浸种时间>染色温度;测定种子生活力的最适条件为种子浸泡12 h后,30℃下0.3%TTC染色液染色15 h。In order to realize the rapid determination of the seed vigor of Aconitum kusnezoffii,through the orthogonal test of 4 factors and 3 levels,the influence of each factor in the seed vigour of Aconitum kusnezoffii on the dyeing effect was explored,and the optimal dyeing conditions were determined.The results showed that the influence of various factors on the seed dyeing effect was ranked as dye⁃ing time>TTC concentration>soaking time>dyeing temperature.The most suitable condition for determining seed viability is after seed soaking for 12 h,staining with 0.3%TTC dye solution at 30℃for 15 h.

关 键 词：北乌头(Aconitum kusnezoffii) 种子 TTC染色法 生活力 

分 类 号：R282[医药卫生—中药学]