共代谢条件下荧蒽降解菌的差异蛋白质组学分析  

Differential proteomic analysis of fluoranthene-degrading bacteria under co-metabolic conditions

作　　者：钟琦 王红旗[1] 姜茹菡 肖雅倩 吴枭雄[1] ZHONG Qi;WANG Hong-qi;JIANG Ru-han;XIAO Ya-qian;WU Xiao-xiong(College of Water Sciences,Beijing Normal University,Beijing 100875,China)

机构地区：[1]北京师范大学水科学研究院,北京100875

出　　处：《中国环境科学》2022年第9期4423-4432,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(41772234)。

摘　　要：以课题组筛选出的多环芳烃高效降解菌--蜡样芽孢杆菌(Bacillus cereus)DQ01菌株为研究对象,以荧蒽为目标污染物,以牛肉膏蛋白胨培养基(LB培养基)为共代谢基质,基于同位素相对和绝对定量技术(iTRAQ)比较细菌在无机盐培养基(MSM培养基)和MSM-LB培养环境中降解荧蒽的全蛋白表达水平,旨在揭示蜡样芽孢杆菌在共代谢过程中分子代谢水平上的变化.结果共检测到64个差异表达蛋白质,其中上调表达的蛋白质有28个,下调表达的蛋白质36个.鉴定到差异蛋白的功能集中在代谢、应激反应、信息传递和调控等方面,同时对比KEGG通路富集分析的结果,也显示细菌有关能量代谢和信息传递、调控的通路产生的变化.比较细菌在是否处于共代谢下的差异蛋白表达,推测细菌在降解多环芳烃过程中的关键环节是小分子代谢和适应外界环境的通路.The research object was the Bacillus cereus sp.DQ01,a highly effective Polycyclic Aromatic Hydrocarbons degrading bacteria screened by the research group.Fluoranthene and Luria-Bertani medium(LB medium)were used as the target pollutant and the co-metabolic substrate,respectively.The whole protein expression result of bacteria degrading fluoranthene in Minimal Salt Medium(MSM)and MSM-LB culture environment was compared with the isobaric tags for relative and absolute quantity(iTRAQ)technology to reveal changes in molecular metabolism level of Bacillus cereus in the process of co-metabolism.In the results,a total of 64 differentially expressed proteins were detected,consisting of 28 up-regulated proteins and 36 down-regulated proteins.It was identified that the functions of differential proteins focused on metabolism,stress response,information transmission and regulation.At the same time,compared to the results from KEGG pathway enrichment analysis,the changes in bacterial pathways were thought to relate to energy metabolism,information transmission and regulation.Compared with the differential protein expression of bacteria under co-metabolism,the key link of bacteria in the process of degrading PAHs could be speculated to be the pathway of small molecule metabolism and adaptation to the external environment.

关 键 词：共代谢 差异蛋白质组学 多环芳烃 微生物降解 蛋白质功能分析 

分 类 号：X172[环境科学与工程—环境科学]