小青龙汤对过敏性鼻炎大鼠Th1/Th2细胞因子失衡及SP、Annexin1、VCAM-1表达的影响  

Effects of Xiaoqinglong Decoction on Th1/Th2 cytokine imbalance and the expressions of SP,Annexin1,VCAM-1 in rats with allergic rhinitis

作　　者：王金凤[1] 王枫[1] 赵桂芝[1] 于兴娟[1] 祝建材[1] WANG Jinfeng;WANG Feng;ZHAO Guizhi;YU Xingjuan;ZHU Jiancai(Shandong College of Traditional Chinese Medicine,Yantai 264199,Shandong,China)

机构地区：[1]山东中医药高等专科学校,山东烟台264199

出　　处：《现代中西医结合杂志》2022年第15期2051-2057,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：山东省中医药科技发展计划项目(2015-220)。

摘　　要：目的探讨小青龙汤对过敏性鼻炎大鼠Th1/Th2细胞因子失衡及P物质(SP)、膜联蛋白1(Annexin1)、血管细胞黏附分子-1(VCAM-1)表达的影响。方法选取SPF级SD大鼠48只,采用随机数字表法分为正常组、模型组、苍耳子鼻炎胶囊组和小青龙汤高、中、低剂量组,每组8只。正常组大鼠给予常规饲养,其余5组大鼠采用卵白蛋白腹腔注射致敏、双侧鼻腔滴鼻激发方法复制过敏性鼻炎模型。模型复制成功后,苍耳子鼻炎胶囊组大鼠给予苍耳子鼻炎胶囊溶液0.28 g/kg灌胃,小青龙汤高、中、低剂量组分别给予小青龙汤14 g/kg、7 g/kg、3.5 g/kg灌胃,均每日1次,连续灌胃14 d。灌胃结束后,对各组大鼠进行行为学评分,HE染色观察大鼠鼻黏膜组织病理变化,ELISA法检测大鼠血清免疫球蛋白E(IgE)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)水平,免疫组化法检测大鼠鼻黏膜组织中SP、Annexin1、VCAM-1表达情况。结果与模型组比较,小青龙汤高、中剂量组和苍耳子鼻炎胶囊组大鼠行为学评分明显降低(P均<0.05),鼻黏膜病理改变减轻,血清IgE、IL-4水平及鼻黏膜组织中SP、VCAM-1平均光密度值均明显降低(P均<0.05),血清IFN-γ水平及鼻黏膜组织中Annexin1平均光密度值均明显升高(P均<0.05);与小青龙汤中剂量组比较,小青龙汤高剂量组和苍耳子鼻炎胶囊组大鼠行为学评分均更低(P均<0.05),鼻黏膜病理改变更轻,血清IgE、IL-4水平及鼻黏膜组织中SP、VCAM-1平均光密度值均更低(P均<0.05),血清IFN-γ水平及鼻黏膜组织中Annexin1平均光密度值均更高(P均<0.05);小青龙汤高剂量组与苍耳子鼻炎胶囊组上述指标比较差异均无统计学意义(P均>0.05)。结论小青龙汤可呈剂量依赖性改善过敏性鼻炎大鼠的鼻黏膜炎性症状,其作用机制可能与抑制大鼠炎症介质SP、VCAM-1表达,上调Annexinl表达,调节Th1/Th2细胞因子失衡有关。Objective It is to explore the effect of Xiaoqinglong Decoction on Th1/Th2 cytokine imbalance and the expressions of substance P(SP),Annexin1,vascular cell adhesion molecule-1(VCAM-1)in rats with allergic rhinitis(AR).Methods Forty-eight SPF SD rats were divided into 6 groups by random number table method,including normal group,model group,Cangerzi rhinitis capsule group,Xiaoqinglong Decoction high-dose,medium-dose and low-dose groups,with 8 rats in each group.The rats in the normal group were given routine feeding,and the rats in the other 5 groups were sensitized by intraperitoneal injection of ovalbumin(OVA)and stimulated by bilateral nasal drops to establish the models of allergic rhinitis.After the models were established successfully,the rats in Cangerzi rhinitis capsule group were given Cangerzi rhinitis capsule solution 0.28 g/kg by intragastric administration,the rats in Xiaoqinglong Decoction high-dose,medium-dose and low-dose groups were respectively given Xiaoqinglong Decoction 14 g/kg,7 g/kg,3.5 g/kg by intragastric administration,all once per day for 14 days.After the intervention,behavioral scores of the rats in each group were evaluated,the pathological changes in nasal mucosa tissues were observed by HE staining,the level of immunoglobulin E(IgE),interleukin 4(IL-4),interferon-γ(IFN-γ)were detected by enzyme linked immunosorbent assay(ELISA),the expression levels of SP,Annexin1,VCAM-1 in nasal mucosa of the rats were detected by immunohistochemistry.Results Compared with the model group,the behavioral scores of the rats in Xiaoqinglong Decoction high-dose,medium-dose groups and Cangerzi rhinitis capsule group were significantly decreased(all P<0.05),pathological changes in the nasal mucosa were relieved,the levels of IgE and IL-4 in serum and the mean optical density(MOD)of SP,VCAM-1 in nasal mucosa of the rats were significantly decreased(all P<0.05),the level of IFN-γand the MOD of Annexin1 were significantly increased(all P<0.05).Compared with Xiaoqinglong Decoction medium-dose group,the be

关 键 词：小青龙汤 过敏性鼻炎 P物质 膜联蛋白1 血管细胞黏附分子-1 TH1/TH2细胞因子 

分 类 号：R-332[医药卫生]