Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling  

作　　者：Bo-Ra Jeon Muhammad Irfan Seung Eun Lee Jeong Hoon Lee Man Hee Rhee 

机构地区：[1]Laboratory of Physiology and Cell Signaling,College of Veterinary Medicine,Kyungpook National University,Daegu,41566,Republic of Korea [2]Department of Herbal Crop Research,National Institute of Horticultural and Herbal Science(NIHHS),Eumseong,27709,Republic of Korea

出　　处：《Chinese Journal of Integrative Medicine》2022年第9期802-808,共7页中国结合医学杂志（英文版）

基　　金：Supported by a grant of National Research Foundation of Korea(No.2018R1D1A1A09083797)。

摘　　要：Objective:To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract.Methods:Standard light aggregometry was used for platelet aggregation,intracellular calcium mobilization assessed using Fura-2/AM,granule secretion(ATP release)by luminometer,and fibrinogen binding to integrin α_(Ⅱb)β_(3) detected using flow cytometry.Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase(MAPK)and phosphoinositide 3-kinase(PI3K)/Akt signaling.Results:Rumex acetosella displayed the ability to inhibit platelet aggregation,calcium mobilization,granule secretion,and fibrinogen binding to integrin α_(Ⅱb)β_(3).Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation(all P<0.01).Conclusion:Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling,and it may protect against the development of platelet-related cardiovascular diseases.

关 键 词：ANTIPLATELET ETHNOMEDICINE integrinα_(Ⅱb)β_(3) mitogen-activated protein kinase Rumex acetosella L. 

分 类 号：R285[医药卫生—中药学]