基于导电高分子-叶绿体杂化材料活性氧增强策略的肿瘤治疗  

Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment

作　　者：袁海涛 申奇 于汶 曾悦 白昊天[1] 黄一鸣 吕凤婷[1] 王树[1] Hai-tao Yuan;Qi Shen;Wen Yu;Yue Zeng;Hao-tian Bai;Yi-ming Huang;Feng-ting Lv;Shu Wang(Beijing National Laboratory for Molecular Sciences,Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190)

机构地区：[1]中国科学院化学研究所有机固体重点实验室,北京分子科学国家研究中心,北京100190

出　　处：《高分子学报》2022年第10期1251-1260,共10页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号22022705);中国科学院王宽诚教育基金(基金号GJTD-2020-02)资助项目.

摘　　要：构建了一种基于共轭聚合物/叶绿体生物杂化材料的肿瘤治疗新方法.共轭聚合物侧链带正电荷,能够与表面带负电荷的叶绿体通过静电作用结合,从而构造共轭聚合物/叶绿体生物杂化材料.作为非生物成分,共轭聚合物PBF(poly(boron-dipyrromethene-co-fluorene))具有优异的光捕获和光转换能力,不仅提高了叶绿体对太阳光的利用效率,加速叶绿体内光反应中心的电子传递速率;而且进一步介导了光合作用中活性氧(ROS)的定向增强,实现肿瘤细胞的高效清除.基于共轭聚合物/叶绿体生物杂化材料的肿瘤治疗方法为肿瘤治疗提供了一种新思路.A new antitumor treatment based on conjugated polymer/chloroplast biological hybrid material was proposed in this study,in which the conjugated polymer PBF(poly(boron-dipyrromethene-co-fluorene))with a positive side chain charge could be electrostatically combined with negatively charged chloroplast.The successful combination of conjugated polymer PBF and chloroplast was demonstrated by the UV-Vis absorption spectra,the zeta potentials,and isothermal titration microcalorimetry(ITC)in sequence.In addition,the confocal laser scanning microscopy(CLSM)and scanning electron microscopy(SEM)were utilized to visualize the structure of the PBF/chloroplast biological hybrid.The CLSM results demonstrated that the conjugated polymer PBF was closely surrounded by the chloroplast.The SEM image showed the PBF/chloroplast biological hybrid material was much rougher than that of the pristine chloroplast on account of the coating behavior of conjugated polymer PBF.As an abiotic component,the conjugated polymer PBF possessed excellent light-harvesting and light conversion capability,which not only improved the utilization efficiency of sunlight in chloroplasts but also accelerated the electron transfer rate of the light reaction center in chloroplasts.The estimation was studied by the NADPH level of the live chloroplast.Under white light illumination,the NADPH in PBF/chloroplast was significantly increased compared to that of the pristine chloroplast.This increase in NADPH indicates that the photoinduced electron transfer by conjugated polymer PBF could improve the efficiency of NADPH-related intracellular reactions.The acceleration of the photosynthetic rate was also investigated through detecting the reduction rate of 2,6-dichlorophenolindophenol(DCPIP),an artificial electron acceptor,which would capture the transported electrons of the chloroplast during the light reaction.Moreover,it further mediated the directional enhancement of reactive oxygen species(ROS)in photosynthesis to realize the efficient clearance of tumor cells.Ov

关 键 词：共轭聚合物 叶绿体 光合作用 活性氧增强 肿瘤治疗 

分 类 号：R730.5[医药卫生—肿瘤]