微小RNA-203预测胃癌肝转移及预后的临床价值  

Clinical value of microRNA-203 in predicting liver metastasis and prognosis of gastric cancer

作　　者：姜孝奎[1] 李振想[1] 孟鑫[1] 李韶山[1] JIANG Xiaokui;LI Zhenxiang;MENG Xin;LI Shaoshan(Department of General Surgery,The Third Affiliated Hospital of Xinxiang Medical College,Xinxiang,Henan 453000,China)

机构地区：[1]新乡医学院第三附属医院普外科,河南新乡453000

出　　处：《安徽医药》2022年第10期2009-2012,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的检测胃癌病人血清中循环微小RNA-203(miR-203)对胃癌肝转移和预后的评估价值。方法收集2015年1月至2017年12月在新乡医学院第三附属医院接受手术治疗的胃癌病人115例,非胃癌病人40例。qRT-PCR检测胃癌病人和非胃癌病人外周血中miR-203的表达水平,并比较胃癌病人不同病理特征组间miR-203的表达水平。logistic单因素和多因素回归分析影响胃癌肝转移的危险因素,Cox单因素和多因素回归分析影响胃癌术后3年死亡的危险因素,受试者工作曲线(ROC)检测外周血中miR-203预测胃癌肝转移和胃癌术后3年死亡的临床价值。结果胃癌病人外周血中miR-203的表达水平为0.482±0.107,低于非胃癌病人外周血中的水平1.529±0.154(t=8.20,P<0.001)。胃癌病人的区域淋巴结(N)分期、远处转移(M)分期、原发肿瘤区、区域淋巴结、远处转移(TNM)分期、肝转移、腹膜转移和术后3年存活情况与miR-203的表达水平有关(P<0.05)。N分期、M分期、TNM分期和miR-203为影响胃癌肝转移的独立危险因素。N分期、M分期、TNM分期、肝转移、腹膜转移和miR-203为影响胃癌术后3年内死亡的独立危险因素。外周血中miR-203预测胃癌肝转移的曲线下面积(95%CI)为0.91(0.86,0.92),特异度为81.4%,灵敏度为80.3%,预测胃癌术后3年内病人死亡的曲线下面积(95%CI)为0.85(0.81,0.89),特异度为72.4%,灵敏度为89.3%,均具有一定临床价值。结论胃癌外周血中miR-203水平的降低对肝转移和术后3年死亡具有一定的临床预测价值。Objective To detect the value of circulating microRNA-203(miR-203)in the serum of patients with gastric cancer in evaluating liver metastasis and prognosis of gastric cancer.Methods A total of 115 gastric cancer patients and 40 non-gastric cancer patients who underwent surgery in The Third Affiliated Hospital of Xinxiang Medical College from January 2015 to December 2017 were enrolled in the study.The expression levels of miR-203 in peripheral blood of gastric cancer patients and non-gastric cancer patients were detected by qRT-PCR,and the expression levels of miR-203 in different pathological groups of patients with gastric cancer was compared.Logistic univariate and multivariate regression analysis were used to analyze the risk factors for liver metastasis of gastric cancer,Cox univariate and multivariate regression analysis were used to analyze the risk factors for 3-year postoperative death of gastric cancer,and receiver operating curve(ROC)was used to detect the clinical value of miR-203 in peripheral blood to predict liver metastasis and 3-year postoperative death of gastric cancer.Results The expression level of miR-203 in peripheral blood of patients with gastric cancer was 0.482±0.107,which was lower than the level of 1.529±0.154 in peripheral blood of patients with non-gastric cancer(t=8.20,P<0.001).The expression of miR-203 was correlated with Lymph node(N)staging,distant metastasis(M)staging,primary tumour area,regional lymph nodes,TNM staging,liver metastasis,peritoneal metastasis and 3-year survival of gastric cancer patients(P<0.05).Lymph node(N)staging,distant metastasis(M)staging,TNM staging,and miR-203 were independent risk factors for liver metastasis of gastric cancer.N staging,M staging,TNM staging,liver metastasis,peritoneal metastasis and miR-203 were independent risk factors for death within 3 years after gastric cancer surgery.The area under the curve(95%CI)of miR-203 in peripheral blood for predicting liver metastasis of gastric cancer was 0.91(0.86,0.92),the specificity was 81.4%,and

关 键 词：胃肿瘤 肝转移 循环微小RNA-203 预后 

分 类 号：R735.2[医药卫生—肿瘤]