非洲猪瘟病毒内囊膜蛋白p17与宿主互作蛋白的初步鉴定  

Identification of host proteins interacting with African swine fever virus inner envelope protein p17

作　　者：钟桂芳 邓婷娟 徐康[1] 倪温碧 王裴 胡伯里[1] 周继勇[1] ZHONG Guifang;DENG Tingjuan;XU Kang;NI Wenbi;WANG Pei;HU Boli;ZHOU Jiyong(MOA Key Laboratory of Animal Virology/Center of Veterinary Science,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学动物医学中心/农业农村部动物病毒学重点实验室,浙江杭州310058

出　　处：《生物工程学报》2022年第8期2883-2890,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(31941009)。

摘　　要：非洲猪瘟病毒(African swine fever virus,ASFV)的感染导致猪的死亡率高达100%,给养猪业造成毁灭性灾难。因此,开展针对ASFV感染复制的研究有着重大的意义。目前发现ASFV有超过150个开放阅读框,其中D117L基因编码的内囊膜蛋白p17参与病毒二十面体结构的形成,但是对p17调控宿主细胞功能的机制知之甚少。研究通过免疫沉淀技术联合蛋白质谱分析,初步筛选出与ASFV p17潜在的宿主互作蛋白。通过进一步免疫共沉淀技术和激光共聚焦实验确认了p17与线粒体外膜蛋白TOMM70(translocase of outer mitochondrial membrane 70)、热休克蛋白HSPA8(heat shock 70 kDa protein 8)的互作。该研究为进一步探索p17在ASFV感染过程中的功能提供了重要信息。African swine fever virus(ASFV)infection leads to a mortality rate of up to 100%,causing devastating disasters to the pig industry.Understanding the ASFV infection and replication is therefore of great importance.ASFV has more than 150 open reading frames,among which the inner coat protein p17 encoded by the D117L gene is involved in the formation of the icosahedral structure of the virus.However,little is known about the mechanism how p17 regulates host cell function.In this study,the potential host proteins interacting with ASFV p17 were screened by immunoprecipitation technique combined with protein profiling analysis.The interactions of p17 with mitochondrial membrane protein TOMM70 and heat shock protein HSPA8 were confirmed by co-immunoprecipitation technique and laser confocal experiments.This study provides important information for further exploring the function of p17 during ASFV infection.

关 键 词：非洲猪瘟病毒 p17 免疫沉淀技术 质谱 互作蛋白 

分 类 号：S852.65[农业科学—基础兽医学]