地衣芽孢杆菌分批补料发酵γ-聚谷氨酸  

Fed-batch Fermentation of γ-Polyglutamate by Bacillus licheniformis

作　　者：郭建敏 郭利飞 赵廷彬 李镇江 江源刚 叶莉 唐晓芳 杨志刚 乔长晟[1,2,3] GUO Jian-min;GUO Li-fei;ZHAO Ting-bin;LI Zhen-jiang;JIANG Yuan-gang;YE Li;TANG Xiao-fang;YANG Zhi-gang;QIAO Chang-sheng(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Key Laboratory of Industrial Microbiology,Education Ministry Key Laboratory of Industrial Fermentation Microbiology,Tianjin 300457,China;Tianjin Huizhi Biotrans Biotechnology Co.,Ltd.,Tianjin 300457,China;Sichuan Baichuan Jinkai Biological Engineering Co.,Ltd.,Chengdu 610000,Sichuan,China;Sichuan Food Fermentation Industry Research and Design Institute Co.,Ltd.,Chengdu 610000,Sichuan,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市工业微生物重点实验室,工业发酵微生物教育部重点实验室,天津300457 [3]天津慧智百川生物技术有限公司,天津300457 [4]四川百川金开生物工程有限公司,四川成都610000 [5]四川省食品发酵工业研究设计院有限公司,四川成都610000

出　　处：《食品研究与开发》2022年第18期191-198,共8页Food Research and Development

基　　金：四川省科技计划重点研发项目(2021YFN0135)。

摘　　要：γ-聚谷氨酸(γ-polyglutamic acid,γ-PGA)是一种应用于食品、农业、医药等领域的生物聚合物。在不补料发酵γ-PGA过程中,存在因培养基中碳源、氮源不足导致菌体生长发育和γ-PGA合成受限的情况。为实现γ-PGA高产,采用分批补料发酵方式补充菌体生长代谢所需的碳源和氮源,在5 L发酵罐中进行γ-PGA分批补料发酵优化,并在200 L发酵罐进行放大验证。结果表明:当培养基中葡萄糖含量低于5 g/L、氨氮浓度低于0.5 g/L时开始流加补料,持续补料12 h将培养基中葡萄糖浓度维持在5 g/L~15 g/L,氨氮浓度维持在0.5 g/L~1.0 g/L。与不补料发酵相比,这一优化使得菌种指数生长期延长了6 h,生物量(OD_(660))达到了0.62,提升了39.01%,谷氨酸含量降至16 g/L,谷氨酸利用率提升了38.47%,γ-PGA生产强度和产量分别为15.69 g/(L·d)、(47.09±0.82)g/L,均提高了38.45%,为γ-PGA工业化生产提供了技术支撑。γ-Polyglutamic acid(γ-PGA)is a type of biopolymer used in food,agriculture,and medicine.In the batch fermentation without feeding process ofγ-PGA,the growth and development of the bacteria and the synthesis ofγ-PGA were restricted by insufficient carbon and nitrogen sources in the medium.To obtain a high yield ofγ-PGA,the fed-batch fermentation method was used to supplement the carbon and nitrogen sources required for bacterial growth and metabolism.γ-PGA fed-batch fermentation was optimized in a 5 L fermenter,with amplification in a 200 L fermenter.The experimental results showed that when the glucose and ammonia nitrogen concentrations in the medium were lower than 5 g/L and 0.5 g/L,respectively,the feed was started;the glucose and ammonia nitrogen concentrations were maintained at 5 g/L-15 g/L and 0.5 g/L-1.0 g/L,respectively,for 12 h.Compared with conventional batch fermentation,this optimized method extended the exponential growth period by 6 h,and the biomass(OD_(660))reached 0.62(39.01%increase vs.control).The glutamate level was reduced to 16 g/L,and the glutamate utilization rate was increased by 48.38%.The productivity and yield ofγ-PGA were 15.69 g/(L·d)and(47.09±0.82)g/L,respectively,demonstrating an increase of 38.45%.This study provides useful information for enhancing the industrial production ofγ-PGA.

关 键 词：地衣芽孢杆菌 发酵优化 Γ-聚谷氨酸 分批补料 谷氨酸利用率 葡萄糖 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]