牙龈卟啉单胞菌GroEL对人脐静脉内皮细胞影响的研究  

Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells

作　　者：闫锐 郭凯利 曹丰弟 张军 马哲[1] YAN Rui;GUO Kaili;CAO Fengdi;ZHANG Jun;MA Zhe(School and Hospital of Stomatology,Shijiazhuang 050017,China;Hebei Key Laboratory of Stomatology,Hebei Medical Uiversity,Shijiazhuang 050017,China;The University of Melbourne of Dentistry,Melbourne VIC 3010,Australia)

机构地区：[1]河北医科大学口腔医学院•口腔医院,河北石家庄050017 [2]河北省口腔医学重点实验室,河北石家庄050017 [3]墨尔本大学牙学院,澳大利亚墨尔本VIC 3010

出　　处：《口腔医学研究》2022年第9期872-876,共5页Journal of Oral Science Research

基　　金：京津冀医疗卫生协调发展和公立医院改革能力建设(编号:2019061636)。

摘　　要：目的:探究牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)的代谢产物GroEL对体外培养的人脐静脉内皮细胞(HUVEC)增殖活性、凋亡及炎症反应的影响,以期为探讨P.g致心血管疾病的发病机制提供理论依据。方法:用不同浓度的P.g GroEL(2、4、6、8、10μg/mL)处理HUVEC 24 h,观察处理前后细胞形态的变化。通过CCK-8法检测HUVEC的增殖活性,流式细胞学检测细胞凋亡,RT-qPCR和ELISA法分别检测白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、细胞间粘附分子-1(ICAM-1)、血管细胞粘附因子-1(VCAM-1)、E-选择素(E-selectin)的表达。结果:P.g GroEL处理HUVEC 24 h后,显著降低了细胞活力并增加了细胞凋亡率(P<0.05),且随着P.g GroEL浓度的增加,对细胞活性的抑制作用和细胞凋亡数量也逐渐增加。P.g GroEL还显著刺激了HUVEC分泌IL-6、IL-8、ICAM-1、VCAM-1、E-selectin(P<0.05)。结论:P.g GroEL以浓度依赖性方式抑制HUVEC活性并诱导细胞发生凋亡。P.g GroEL还能引起HUVEC的炎症反应,从而在牙周炎促进动脉粥样硬化的发生中发挥重要作用。Objective: To investigate the effects of GroEL, a metabolite of Porphyromonas gingivalis(P.g), on the proliferation viability, apoptosis, and inflammation response of human umbilical vein endothelial cell(HUVEC) in vitro. Methods: The HUVEC were stimulated with different concentrations of P.g GroEL(2, 4, 6, 8, 10 μg/mL) for 24 h to observe the morphologic changes. The proliferation viability of HUVEC was detected by CCK-8. Apoptosis rate of HUVEC was detected by flow cytometry. The expressions of interleukin-6(IL-6), interleukin-8(IL-8), intracellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and E-selectin were detected by RT-qPCR and ELISA, respectively. Results: P.g GroEL significantly decreased HUVEC viability, and apoptosis rate was increased at 24 h(P<0.05). The inhibitory effect of P.g GroEL and apoptosis rate was more obvious as the concentration increased. P.g GroEL also promoted the expression of IL-6, IL-8, ICAM-1, VCAM-1, and E-selectin(P<0.05). Conclusion: P.g GroEL may play an important role in the development of atherosclerosis promoted by periodontitis.

关 键 词：牙龈卟啉单胞菌 GROEL 牙周炎 人脐静脉内皮细胞 动脉粥样硬化 

分 类 号：R781.42[医药卫生—口腔医学]