外源植物激素对‘赤霞珠’葡萄冬芽萌发的影响  

Effects of Exogenous Plant Hormones on Germination of Grape Winter Buds

作　　者：张鸿雁 陈莉[1,2,3] 屈嘉燕 李少华[1,2,3] 曾青青 张鹏 管雪强[4,5] 张克坤 房玉林 陈可钦[1,2,3] ZHANG Hongyan;CHEN Li;QU Jiayan;LI Shaohua;ZENG Qingqing;ZHANG Peng;GUAN Xueqiang;ZHANG Kekun;FANG Yulin;CHEN Keqin(College of Enology,Northwest A&F University,Yangling 712100,China;Heyang Grape Experiment Demonstration Station,Northwest A&F University,Heyang 715301,China;The East Foot of Helan Mountain Wine Experiment and Demonstration Station,Northwest A&F University,Yongning 750104,China;Shandong Aeademy of Grape,Jinan 250100,China;Shandong Technology Innovation Center of Wine Grape and Wine,Yantai 265608,China)

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]西北农林科技大学合阳葡萄试验示范站,陕西合阳715300 [3]西北农林科技大学宁夏贺兰山东麓葡萄酒试验示范站,宁夏永宁750104 [4]山东省葡萄研究院,济南250100 [5]山东省酿酒葡萄与葡萄酒技术创新中心,山东烟台265608

出　　处：《中外葡萄与葡萄酒》2022年第5期52-58,共7页Sino-Overseas Grapevine & Wine

基　　金：山东省重点研发计划(2022CXGC010605);国家重点研发计划(2019YFD1002502)财政部和农业农村部:国家农业产业技术体系(CARS-29);宁夏回族自治区重大研发计划(2020BCF01003)。

摘　　要：为进一步揭示不同激素信号对葡萄冬芽萌发的影响,以酿酒葡萄‘赤霞珠’一年生枝条为试材,通过用生长素(ICA)、赤霉素(GA_(7))、脱落酸(ABA)、2,3,5-三碘苯甲酸(TIBA)、多效唑(paclobutrazol)、氟啶酮(fluridone)分别处理冬芽,以清水处理(CK)为对照,研究了不同激素对葡萄萌芽率、芽发育形态以及8个生理指标的影响。结果显示,在冬芽休眠解除过程中,ICA处理组的生理指标成分含量与CK接近;多效唑与氟啶酮处理组生理指标成分含量接近;TIBA处理提高了H_(2)O_(2)含量,使超氧阴离子含量显著下降,SOD酶活性和淀粉含量上升,果糖含量下降,冬芽萌芽率上升;GA_(7)处理下,H_(2)O_(2)和果糖含量下降,SOD酶活性降低,超氧阴离子、淀粉、可溶性糖和可溶性蛋白含量上升,冬芽的萌发受到抑制。以上结果表明,TIBA对葡萄冬芽的萌发有明显的促进作用,而GA_(7)对冬芽的萌发表现出抑制作用,相关结果为深入挖掘葡萄冬芽萌发调控的关键路径奠定了基础。In order to further reveal the influence of different hormone signals on grape winter bud germination,annual branches of'Cabernet Sauvignon'were used as test materials in this study.Winter buds were treated with auxin(ICA),gibberellin(GA_(7)),abscisic acid(ABA)and their corresponding inhibitors 2,3,5-triiodobenzoic acid(TIBA),paclobutrazol,fluridone,respectively,effects of different hormones on the germination rate,buddevelopment morphology and eight internal physiological indexes were investigated.The results showed that,in the process of dormancy release of winter buds,the content of physiological index components in ICA treatment group was close to that of CK,the contents of physiological indexes in paclobutrazole and fludidone treatment groups were close to each other;TIBA treatment increased H_(2)O_(2) content,OFR content decreased significantly,SOD enzyme activity,starch content and germination rate of winter buds increased,fructose content decreased;Treated by GA_(7) treatment,the content of starch,soluble sugar,soluble protein and OFR increased;SOD enzyme activity,content of H_(2)O_(2) and fructose decreased,which inhibited germination of winter buds.The above results showed that TIBA treatment,significantly promoted winter buds germination,while GA_(7) treatment inhibited winter buds germination.The relevant results laid a theoretical foundation for further exploring the key path of grapevine winter bud germination regulation.

关 键 词：赤霞珠 植物激素 冬芽 萌发 

分 类 号：S663.1[农业科学—果树学] S482.8[农业科学—园艺学]