lncRNA LINC00958靶向调控miR-597-5p对乳腺癌MDA-MB-231细胞增殖、迁移、侵袭和凋亡的影响  

The effect of lncRNA LINC00958 targeting and regulating miR-597-5p on the proliferation,migration,invasion and apoptosis of breast cancer MDA-MB-231 cells

作　　者：魏娜[1] 侯净[1] 王光辉 王舒 倪青[1] WEI Na;HOU Jing;WANG Guang-Hui(Breast Surgery,Guizhou Provincial People's Hospital,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州省人民医院乳腺外科,贵州贵阳550002

出　　处：《中国老年学杂志》2022年第18期4488-4495,共8页Chinese Journal of Gerontology

基　　金：国家自然科学基金课题(81660439,81702640);贵州省科技计划项目(黔科合基础[2017]1114)。

摘　　要：目的研究长链非编码RNA(lncRNA)LINC00958影响乳腺癌MDA-MB-231细胞增殖、迁移、侵袭和凋亡的作用与机制。方法定量逆转录聚合酶链反应(qRT-PCR)检测LINC00958和miR-597-5p在乳腺癌组织中的表达。MDA-MB-231细胞转染si-LINC00958或miR-597-5p或共转染si-LINC00958和anti-miR-597-5p。qRT-PCR检测LINC00958和miR-597-5p表达,四甲基偶氮唑蓝(MTT)检测MDA-MB-231细胞增殖,Transwell检测细胞迁移、侵袭,流式细胞术检测细胞凋亡,Western印迹检测Ki-67、基质金属蛋白酶(MMP)-2、MMP-9、B细胞淋巴瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)表达。双荧光素酶报告实验分析LINC00958与miR-597-5p的靶向关系。结果与癌旁组织比较,41例乳腺癌组织中LINC00958表达显著上调,miR-597-5p表达显著下调(P<0.05)。抑制LINC00958表达显著降低MDA-MB-231细胞增殖、Ki-67表达水平、迁移、侵袭细胞数、MMP-2、MMP-9、Bcl-2表达水平,显著提高细胞凋亡率、Bax表达水平(P<0.05),与miR-597-5p过表达的结果相同。LINC00958靶向调控miR-597-5p的表达。干扰miR-597-5p表达后,抑制LINC00958表达对乳腺癌MDA-MB-231细胞增殖、迁移、侵袭和凋亡的作用被显著逆转(P<0.05)。结论LINC00958通过靶向下调miR-597-5p,增强乳腺癌MDA-MB-231细胞的增殖、迁移、侵袭,并减轻细胞凋亡。Objective To study the effect and mechanism of long non-coding RNA(lncRNA)LINC00958 on the proliferation,migration,invasion and apoptosis of breast cancer MDA-MB-231 cells.Methods Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression of LINC00958 and miR-597-5p in breast cancer tissues.MDA-MB-231 cells were transfected with si-LINC00958 or miR-597-5p,or co-transfected with si-LINC00958 and anti-miR-597-5p.qRT-PCR was used to detect the expression of LINC00958 and miR-597-5p,tetramethyl azolium blue(MTT)was adopted to detect the proliferation of MDA-MB-231 cells,Transwell was used to detect cell migration and invasion,flow cytometry was used to detect cell apoptosis,Western blot was adopted to detect Ki-67 and matrix metalloproteinases(MMP)-2,MMP-9,B-cell lymphoma(Bcl-2),Bcl-2 related X protein(Bax)expression.The dual luciferase report experiment was used to analyze the targeting relationship between LINC00958 and miR-597-5p.Results Compared with adjacent tissues,the expression of LINC00958 was significantly up-regulated and the expression of miR-597-5p was significantly down-regulated in 41 breast cancer tissues(P<0.05).Inhibition of LINC00958 expression significantly reduced MDA-MB-231 cell proliferation,Ki-67 expression level,migration,number of invaded cells,MMP-2,MMP-9,Bcl-2 expression levels,and significantly increased cell apoptosis rate and Bax expression level(P<0.05),which was the same as the result of miR-597-5p overexpression.LINC00958 targeted the expression of miR-597-5p.After interfering with the expression of miR-597-5p,the effect of inhibiting the expression of LINC00958 on the proliferation,migration,invasion and apoptosis of breast cancer MDA-MB-231 cells was significantly reversed(P<0.05).Conclusions LINC00958 could enhance the proliferation,migration and invasion of breast cancer MDA-MB-231 cells by targeting down-regulation of miR-597-5p,and reducing cell apoptosis.

关 键 词：LINC00958 miR-597-5p 乳腺癌 增殖 转移 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]