川荞2号表型变异突变体筛选及黄酮代谢相关基因的表达分析  

Identification of Chuanqiao 2 Mutants with Phenotypic Variation and Expression Analysis of Flavonoid-Related Genes

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作  者:彭艳[1] 刘婷婷 阮景军[1] 姚鑫 唐勇 高安静 程剑平[1] PENG Yan;LIU Ting-ting;RUAN Jing-jun;YAO Xin;TANG Yong;GAO An-jing;CHENG Jian-ping(College of Agriculture,Guizhou University,Guiyang 550025;Agriculture and Rural Affairs Bureau of Qianxinan Prefecture,Guizhou Xingyi 562400)

机构地区:[1]贵州大学农学院,贵阳550025 [2]黔西南州农业农村局,贵州兴义562400

出  处:《植物遗传资源学报》2022年第5期1508-1520,共13页Journal of Plant Genetic Resources

基  金:国家重点研发计划“政府间国际科技创新合作/港澳台科技创新合作”重点专项(2017YFE0117600);国家重点研发计划“科技助力经济2020”重点专项(SQ2020YFF0402959);国家自然科学基金(32160669);贵州省科技支撑计划(黔科合支撑[2020]1Y051号)。

摘  要:以50 mmol/L甲磺酸乙酯(EMS,ethyl methanesulfonate)为诱变剂,构建川荞2号的突变体库,并观察了M2至M3群体表型的变化情况,测定M3群体中叶片和茎秆颜色突变植株的叶绿素和花青素含量,并用qRT-PCR对叶片和茎秆颜色突变植株中的黄酮合成代谢酶基因(CHS、F3H、F3′H、FLS和UFGT)进行表达量分析。结果表明,在M2突变群体中发现茎秆、叶片和生育期的突变植株共177株,突变率为10.56%;在M3群体中的突变类型较丰富,包括叶片、生育期、茎秆和其他类型的突变植株共665株,占M3群体的45.86%;同时,也观察到M3籽粒的形状、颜色和大小均发生了不同程度的突变。通过测量发现,叶绿素和花青素含量在两类突变植株中的变化趋势几乎一致,均只有叶基部红色植株的含量高于正常植株,其余类型的突变植株含量均低于正常株,其中黄绿色斑纹叶的含量最低。此外,还发现CHS和F3H基因在红色茎秆植株中的表达量最高,FLS基因在黄色叶片植株中的表达量最高,而F3′H在叶边缘红色植株和叶基部红色植株中的表达量较高,其中在叶边缘红色植株中的含量最高;由此推测,M3群体中叶片和茎秆颜色的突变可能与黄酮合成相关基因的表达有关。本研究筛选的突变体将为下一步的研究工作提供基础材料。In this study,we generated a mutant population of tartary buckwheat cultivar Chuanqiao 2 treated by 50 mmol/L ethyl methanesulfonate(EMS)solution.We recorded the phenotypic variations of M_(2) to M_(3) lines,and also measured the content of chlorophyll and anthocyanin in the mutant plants with leaf and stem color variation in the M_(3) population as well as the transcription level of flavonoid synthesis and metabolite related genes(CHS,F3H,F3'H,FLS and UFGT).177 mutant plants showing alternations on stem and/or leaf morphology and/or growth stages were found in M_(2) population with a mutation rate of 10.56%.665 mutant plants in M_(3) population were found,including leaves,growth stages,stems,and other types of mutant plants,accounting for 45.86%of the M_(3) population.We also observed that the shape,color and size of grains in M_(3) generation changed in varying degrees.We found that the change trend of chlorophyll and anthocyanin content in plants with color variation were almost the same,the content of the red leaf base plant was higher than that of the normal plant,and the content of the other types of mutant plants were lower than that of the normal plant.Among them,the variegated leaf had the lowest content.We also found that CHS and F3H genes had the highest expression level in red stalk plants,the FLS genes had the highest expression level in yellow leaf plants,and the expression of F3'H was higher in the two types of plants with red leaf base and red leaf edge than the other types,and the content was the highest in the plants with red leaf edge.It is speculated that leaf and stem color variations in the M_(3) population may be contributed by the expression of flavonoid synthesisrelated genes.Collectively,these mutants gained from this study will provide the basic material for future research.

关 键 词:苦荞 甲磺酸乙酯 突变体 黄酮合成 基因表达 

分 类 号:S517[农业科学—作物学]

 

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