羊奶冻干粉中恩诺沙星基体标准物质的研制  

Development of a Reference Material for Enrofloxacin in Lyophilized Goat’s Milk

作　　者：刘天和 武彩红[1] 左伟勇[1] 杜汶伸 曹习超 魏宁[1] LIU Tianhe;WU Caihong;ZUO Weiyong;DU Wenshen;CAO Xichao;WEI Ning(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《中国乳品工业》2022年第9期24-28,共5页China Dairy Industry

基　　金：多种禁用抗菌药物残留检测过程中基体标准物质的研发(NSF2021ZR06);2021年江苏省高校大学生创新创业训练计划项目(202112806003Y)。

摘　　要：为了给羊奶中恩诺沙星的残留检测提供实物基体标准物质,并满足在恩诺沙星残留检测过程中质量控制和方法学验证的需求,需要研制一批羊奶冻干粉中恩诺沙星基体标准物质。选取3只健康的泌乳期奶山羊,肌肉注射恩诺沙星后收集羊奶,利用冷冻干燥技术制得一批羊奶冻干粉恩诺沙星基体标准物质。对制备好的羊奶冻干粉恩诺沙星基体标准物质进行均匀性、稳定性评价、定值、不确定度评估。在均匀性评估中,获得的F值1.92<F_(0.05)(14,30),在稳定性评估中,分别进行了短期稳定性实验(4,-20,25℃,2周)和长期稳定性实验(-20、-80℃,12个月).数据表明,羊奶冻干粉恩诺沙星基体标准物质的均匀性、稳定性良好,8家实验室联合定值,制备的标准物质最终定值结果为101.08μg/kg,总不确定度为2.7μg/kg.In order to provide physical matrix reference materials for the detection of enrofloxacin residues in goat milk,and meet the requirements of quality control and methodological verification in the process of enrofloxacin residue detection,a batch of enrofloxacin matrix reference materials in goat milk lyophilized powder needs to be developed.Three healthy lactating dairy goats were selected,goat milk was collected after intramuscular injection of enrofloxacin,and a batch of goat milk freeze-dried powder was prepared by freeze-drying technology.En—rofloxacin matrix standard material.The prepared goat milk freeze-dried powder enrofloxacin matrix standard material was evaluated for homogeneity,stability,fixed value and uncertainty.In the homogeneity evaluation,the obtained F value was 1.92<F_(0.05)(14,30),and in the stability evaluation,short-term stability experiments were performed(4,-20,25℃;2 weeks)and long-term stability experiments(-20℃,-80℃;12 months).The data show that the uniformity and stability of the enrofloxacin matrix standard material of goat milk freeze—dried powder is good.2.7 μg/kg.

关 键 词：标准物质 冷冻干燥 羊奶 恩诺沙星 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]