溃疡性结肠炎患者血清外泌体miRNA-221水平及临床意义  

Expression and Clinical Significance of serum Exosomal miRNA-221 in Patients with Ulcerative Colitis

作　　者：董丽丽[1] 李珍[1] 徐蓉[1] 丁芳[1] 李华铭[1] DONG Lili;LI Zhen;XU Rong(Department of Gastroenterology,Hangzhou Third People′s Hospital,Zhejiang 310009,China)

机构地区：[1]杭州市第三人民医院消化内科,310009

出　　处：《医学研究杂志》2022年第9期97-100,127,共5页Journal of Medical Research

基　　金：浙江省卫生和计划生育委员会科技计划项目(2019KY505);浙江省杭州市科技局计划项目(20201231Y029)。

摘　　要：目的 探讨血清外泌体miRNA-221在溃疡性结肠炎患者中的表达及临床意义。方法 选取2020年1月~2021年1月笔者医院收治的溃疡性结肠炎(UC)患者80例作为病例组,另选取同期60例健康体检者作为对照组。采用定量PCR仪检测血清miRNA-221水平,绘制受试者工作特征(ROC)曲线并计算曲线下面积(AUC)分析miRNA-221对UC患者的诊断价值。结果 病例组红细胞沉降率、基底浆细胞、C反应蛋白(C-reaction protein, CRP)、miRNA-221水平高于对照组(P<0.05)。内镜活动度重度活动组和中度活动组血清miRNA-221水平高于轻度活动组,重度活动组血清miRNA-221水平高于中度活动组(P<0.05)。严重度指数重度和中度患者血清miRNA-221水平高于轻度患者,重度患者血清miRNA-221水平高于中度患者(P<0.05)。血清miRNA-221与红细胞沉降率、基底浆细胞、CRP、Mayo、UCEIS评分呈正相关(P<0.05)。血清miRNA-221在预测发生识别UC、UC内镜下活动度、UC严重程度的ROC曲线下面积(AUC)分别为0.786(95%CI:0.713~0.857)、0.674(95%CI:0.576~0.771)、0.801(95%CI:0.725~0.887)。结论 溃疡性结肠炎血清miRNA-221水平升高,血清miRNA-221水平与溃疡性结肠炎疾病严重程度呈正相关,其表达水平有助于早期识别评估UC的发展阶段和疾病严重程度,可作为早期诊治的生物学标志物。Objective To investigate the expression and clinical significance of serum exosomal miRNA-221 in patients with ulcerative colitis. Methods A total of 80 patients with ulcerative colitis(UC) who were admitted to our hospital from January 2020 to January 2021 were selected as the case group, and 60 healthy subjects in the same period were selected as the control group. A quantitative PCR instrument was used to detect the serum miRNA-221 level. The receiver operating characteristic(ROC) curve and the area under the curve(AUC) were used to analyze the diagnostic value of miRNA-221 for UC patients. Results The red blood cell sedimentation rate, basal plasma cell, CRP, and miRNA-221 expression levels in the case group were significantly higher than those in the control group(P<0.05). The level of serum miRNA-221 in the severely active group and the moderately active group of endoscopic activity was higher than that of the lightly active group, and the serum miRNA-221 level of the severely active group was higher than that of the moderately active group(P<0.05). The serum miRNA-221 level of severe and moderate severity index patients was significantly higher than that of mild patients, and the serum miRNA-221 level of severe patients was higher than that of moderate patients(P<0.05). Serum miRNA-221 level was positively correlated with erythrocyte sedimentation rate, basal plasma cells, CRP, Mayo score, UCEIS score(P<0.05). The area under the ROC curve(AUC) of serum miRNA-221 in predicting the occurrence of UC, UC endoscopic activity, and UC severity were 0.786(95%CI:0.713-0.857) and 0.674(95%CI:0.576-0.771), respectively, 0.801(95%CI:0.725-0.887) respectively. Under the cut-off value of 1.12, the sensitivity of serum miRNA-221 to predict UC was 70.1%, and the specificity was 65.3%. Conclusion The level of serum miRNA-221 in ulcerative colitis is elevated, and the level of serum miRNA-221 is positively correlated with the severity of ulcerative colitis. Its expression level is helpful for early identification and evalua

关 键 词：miRNA-221 溃疡性结肠炎 临床意义 内镜活动度 

分 类 号：R574.62[医药卫生—消化系统]