TCT联合HPV E6/E7 mRNA检测在宫颈上皮内瘤样病变和宫颈浸润癌中的应用价值  

Application value of TCT combined with HPV E6/E7 mRNA detection in cervical intraepithelial neoplasia and invasive cervical carcinoma

作　　者：杨弋弋[1] 周新欢[1] 邹科 倪鸣[1] YANG Yi-yi;ZHOU Xin-huan;ZOU Ke;NI Ming(Department of Gynecology,Nanyang Central Hospital,Nanyang,Henan 473000,China)

机构地区：[1]南阳市中心医院妇科,河南南阳473000

出　　处：《中国卫生检验杂志》2022年第15期1811-1815,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的 探讨薄层液基细胞学检测(TCT)联合人乳头瘤病毒(HPV) E6/E7 mRNA检测在宫颈上皮内瘤样病变(CIN)和宫颈浸润癌(ICC)中的诊断价值及与预后的关系。方法 选取2017年9月—2021年12月本院收治的396例疑似宫颈病变患者作为研究对象,均行TCT、HPV E6/E7 mRNA及阴道镜下活检。确诊患者均行手术治疗,术后对所有患者进行随访,再次行TCT、HPV E6/E7 mRNA及阴道镜下活检,记录患者复发转移情况。结果 两者联合检测诊断和预测患者复发的阳性预测值、特异度、准确度和ROC曲线下面积均高于单独TCT和HPV E6/E7 mRNA检测(P<0.05)。TCT联合HPV E6/E7 mRNA检测阳性患者中位无复发生存时间低于阴性患者(P<0.05)。结论 TCT联合HPV E6/E7 mRNA检测在诊断CIN和ICC时以及预测患者复发时具有更高的特异性,能够有效提高准确率,具有更大的临床价值。两者联合检测阳性患者预后差于阴性患者。Objective To investigate the diagnostic value and its relationship with prognosis of thinprep cytology test(TCT)combined with human papillomavirus(HPV) E6/E7 mRNA detection in cervical intraepithelial neoplasia(CIN) and invasive carcinoma of cervix(ICC).Methods A total of 396 patients with suspected cervical lesions admitted to our hospital from September 2017 to December 2021 were selected as the research objects,and all underwent TCT,HPV E6/E7 mRNA and colposcopy biopsy.All the confirmed patients received surgical treatment,and all the patients were followed up after surgery.TCT,HPV E6/E7 mRNA and colposcopy biopsy were performed again to record the recurrence and metastasis of the patients.Results The positive predictive value,specificity,accuracy and area under ROC curve of the combined detection of the two in the diagnosis and prediction of the patient.s relapse were higher than those detected by TCT and HPV E6/E7 mRNA alone(P<0.05).The median relapse-free survival time in patients who were positive for both TCT and HPV E6/E7 mRNA was lower than the median relapse-free survival time in patients who were negative(P< 0.05).Conclusion TCT combined with HPV E6/E7 mRNA detection has higher specificity in the diagnosis of CIN and ICC and the prediction of patient recurrence,which can effectively improve the accuracy and has greater clinical value.The prognosis of patients with positive combined detection of the two methods is worse than that of patients with negative combined detection.

关 键 词：宫颈癌 宫颈上皮内瘤样病变 薄层液基细胞学检测 人乳头瘤病毒 诊断价值 预后 

分 类 号：R737.33[医药卫生—肿瘤]