假木豆提取物对四氯化碳致大鼠肝纤维化的保护作用  

Protective effect of Jiamudou extract on hepatic fibrosis induced by carbon tetrachloride

作　　者：姚平[1] 周李煜 夏星[2] 林国彪[2] YAO Ping;ZHOU Liyu;XIA Xing;LIN Guobiao(Department of Pathology,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530001,Guangxi Zhuang Nationality Autonomous Region,China;Department of Pharmacology,Guangxi University of Traditional Chinese Medicine,Nanning 530001,Guangxi Zhuang Nationality Autonomous Region,China)

机构地区：[1]广西中医药大学附属瑞康医院病理科,广西南宁530001 [2]广西中医药大学药理学教研室,广西南宁530001

出　　处：《新乡医学院学报》2022年第9期810-815,共6页Journal of Xinxiang Medical University

基　　金：广西一流学科建设专项经费资助(编号:2019XK085);广西中医药大学重点实验室子课题(编号:J16073)。

摘　　要：目的探讨假木豆提取物抗四氯化碳(CCl_(4))所致大鼠肝纤维化的作用机制。方法将60只Sprague Dawley大鼠随机分为空白组、模型组、水飞蓟素组、低剂量假木豆组、中剂量假木豆组和高剂量假木豆组,每组10只。水飞蓟素组、低剂量假木豆组、中剂量假木豆组、高剂量假木豆组和模型组大鼠通过背部皮下注射CCl_(4)建立肝纤维化模型,空白组大鼠背部皮下注射花生油,每隔2 d注射1次,共8周。从第3周起,水飞蓟素组大鼠每日灌服水飞蓟素胶囊水溶液(50 mg·kg^(-1))1次;假木豆低、中、高剂量组大鼠每日灌服12.5、25.0、50.0 g·L^(-1)的假木豆水提液(20 mL·kg^(-1));空白组和模型组大鼠灌服等体积的蒸馏水;各组大鼠均连续给药6周。观察并记录各组大鼠饮食活动情况、体质量、毛色光亮程度、精神状况等一般情况。末次给药1 h后采集各组大鼠腹主动脉血5 mL,采用酶联免疫吸附试验检测各组大鼠血清中白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)和转化生长因子-β_(1)(TGF-β_(1))水平。采血后处死大鼠,取肝脏,观察肝脏大体形态,然后取大鼠肝脏左叶,制备肝组织切片,行苏木精-伊红染色,于光学显微镜下观察大鼠肝脏病理形态学变化。结果空白组大鼠饮食正常,活动频繁,毛色洁白有光泽,精神状况正常。模型组大鼠饮食减少,活动量明显减少,毛色暗淡、不清洁,精神状况萎靡;水飞蓟素组大鼠毛色欠缺光泽但较清洁,其余一般情况同空白组;假木豆各剂量组大鼠一般情况较模型组有改善,而较空白组差。模型组、水飞蓟素组、低剂量假木豆组、中剂量假木豆组、高剂量假木豆组大鼠体质量增长量少于空白组(P<0.05),低剂量假木豆组大鼠体质量增长少于模型组(P<0.05)。模型组、水飞蓟素组、低剂量假木豆组、中剂量假木豆组、高剂量假木豆组大鼠血清中IL-1β、IL-6、TNF-α水平均高�Objective To explore the mechanism of Jiamudou extract anti-hepatic fibrosis induced by carbon tetrachloride(CCl_(4))in rats.Methods Sixty Sprague Dawley rats were randomly divided into blank group,model group,silymarin group,low-dose Jiamudou group,middle-dose Jiamudou group and high-dose Jiamudou group,with 10 rats in each group.The rats in the model group,silymarin group,low-dose Jiamudou group,middle-dose Jiamudou group and high-dose Jiamudou group were injected subcutaneously with CCl_(4) every 2 days for 8 weeks to establish the liver fibrosis model;the rats in the blank group were injected subcutaneously with peanut oil every 2 days for 8 weeks.From the third week,the rats in the silymarin group were given silymarin capsule aqueous solution(50 mg·kg^(-1)),once a day;the rats in the low-dose Jiamudou group,middle-dose Jiamudou group and high-dose Jiamudou group were given 12.5,25.0,50.0 g·L^(-1) of Jiamudou water extract(20 mL·kg^(-1))by gavage,respectively;the rats in the blank group and model group were given equal volume of distilled water;all rats were continuously administered for 6 weeks.The general conditions such as diet and activity,body weight,hair color brightness and mental state of rats in each group were observed and recorded.One hour after the last administration,5 mL of abdominal aorta blood of rats in each group was collected,and the levels of serum interleukin(IL)-1β,IL-6,tumor necrosis factor-α(TNF-α)and transforming growth factor-β_(1)(TGF-β_(1))were detected by enzyme-linked immunosorbent assay.The rats were killed after blood collection,and the liver was taken to observe the general morphology.Then,the left lobe of liver was taken,and the liver tissue sections were prepared and stained with hematoxylin-eosin.The pathological changes of the liver tissues of rats were observed under the light microscope.Results The rats in the blank group had normal diet,frequent activities,white and shiny hair,and normal mental status.In the model group,the diet of rats was reduced,the activity

关 键 词：假木豆提取物 肝纤维化 白细胞介素-1Β 白细胞介素-6 肿瘤坏死因子-α 转化生长因子-β_(1) 

分 类 号：R575[医药卫生—消化系统]