HPLC快速测定血液制品中的保护剂甘氨酸  

Rapid determination of the protective agent glycine in blood products by HPLC

作　　者：王欢 梁雅晶 宿艳笋[2] 倪晓玉 蒋晔[1] WANG Huan;LIANG Yajing;SU Yansun;NI Xiaoyu;JIANG Ye(College of Pharmacy of Hebei Medical University,Shijiazhuang,Hebei,050017 P.R.China;Hebei Da’an Pharmaceutical Limited Company,Shijiazhuang,Hebei,050220 P.R.China)

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]河北大安制药有限公司,河北石家庄050220

出　　处：《华西药学杂志》2022年第4期417-420,共4页West China Journal of Pharmaceutical Sciences

基　　金：河北省自然科学基金资助项目(H2021206299);大学生创新性实验项目(USIP2021187;USIP2021079)。

摘　　要：目的采用HPLC法测定血液制品中保护剂甘氨酸的含量。方法采用N-羟基琥珀酰亚胺苯甲酸酯进行衍生结合中空纤维离心超滤-HPLC法快速测定甘氨酸。采用Angla Innoval C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.05%三乙胺磷酸缓冲液(25∶75,pH5.0),流速1 mL·min^(-1),检测波长236 nm,进样量10μL。结果40~140 mg·L^(-1)甘氨酸与峰面积呈良好的线性关系(r^(2)>0.9999);几种血液制品的回收率为98.4%~101.8%,RSD<2%。结论所用方法操作简单、快速,结果准确、可靠,可为血液制品中甘氨酸的快速含量测定提供可靠的分析平台。OBJECTIVE To determine the protective agent glycine in blood products by HPLC.METHODS Glycine was rapidly determined by N-hydroxysuccinimide benzoate derivation combined with hollow fiber centrifugal ultrafiltration and high performance liquid chromatography(HPLC).Angla Innoval C_(18) column(150 mm×4.6 mm,5μm)was used with methanol-0.05%triethylamine phosphoric acid buffer(25∶75,pH5.0)as mobile phase at the flow rate of 1 mL·min^(-1).The detection wavelength was 236 nm and sample volume was 10μL.RESULTS The linearity of glycine was good in the range of 40^(-1)40 mg·L^(-1)(r^(2)>0.9999);The recoveries of several blood products were 98.4%^(-1)01.8%with RSD<2%.CONCLUSION The method is simple,rapid,accurate and reliable,and provides a reliable platform for the rapid determination of glycine in blood products.

关 键 词：血液制品 甘氨酸 柱前衍生化 N-羟基琥珀酰亚胺苯甲酸酯 高效液相色谱法 中空纤维离心超滤 含量测定 保护剂 

分 类 号：R917[医药卫生—药物分析学]