分泌粒蛋白Ⅲ(Scg3)抗体对角膜新生血管的抑制作用  

Inhibitory effect of secretogranin Ⅲ antibody on corneal neovascularization

作　　者：靳荷 杨彬彬 蒋冬冬 郑柳 丁芝祥[1] 陆素青[1] JIN He;YANG Binbin;JIANG Dongdong;ZHENG Liu;DING Zhixiang;LU Suqing(Affiliated Hospital of Guilin Medical University,Guilin 541000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]桂林医学院附属医院眼科,广西壮族自治区桂林市541000

出　　处：《眼科新进展》2022年第9期680-684,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金项目(编号:82160197);广西自然科学青年基金项目(编号:2018GXNSFBA050055);广西科技基地和人才专项项目(编号:桂科AD19110131)。

摘　　要：目的分析分泌粒蛋白III(Scg3)抗体对兔角膜新生血管(CNV)的干预作用。方法选取健康新西兰白兔45只,未做碱烧伤的5只作为对照组,剩余40只白兔用碱烧伤法构建CNV模型,随机分为模型组(20只)和干预组(20只),自碱烧伤之日起每隔2 d结膜下分别注射0.1 mL 1×PBS溶液或0.5 mg·L^(-1)的Scg3抗体工作液。碱烧伤后14 d,Western blot检测对照组与模型组兔角膜组织中Scg3的蛋白表达。分别在碱烧伤后1 d、7 d、14 d应用眼前段分析仪测量CNV的面积和长度,应用角膜共聚焦显微镜观察眼表变化,利用蛋白芯片法检测细胞间黏附分子-1(ICAM-1)、白细胞介素-10(IL^(-1)0)、转化生长因子-β1(TGF-β1)等炎症相关细胞因子的表达。结果角膜碱烧伤后14 d,模型组兔烧伤区域出现大量CNV,而干预组兔角膜无明显CNV生长。Western blot检测结果显示,与对照组相比,模型组兔角膜中Scg3蛋白表达显著升高(P<0.05)。角膜共聚焦显微镜下观察可见,模型组兔的角膜缘内出现大量活动性CNV。角膜碱烧伤后7 d和14 d,干预组兔角膜中CNV的长度和面积均显著低于模型组(均为P<0.001)。蛋白芯片法检测结果发现,碱烧伤后7 d、14 d,干预组兔眼表泪液中ICAM-1、IL^(-1)0、TGF-β1等炎症因子表达均显著低于模型组(均为P<0.05)。结论0.5 mg·L^(-1)的Scg3抗体能够降低兔角膜碱烧伤后的炎症反应,抑制CNV的生长。Objective To explore the effect of secretogranin III(Scg3)antibody on rabbit corneal neovascularization(CNV).Methods A total of 45 healthy New Zealand white rabbits were selected in the study,among which 5 rabbits without alkali burns were classified into the control group,and the rest 40 rabbits were used to establish CNV models with alkali burns and then were randomly divided into the model group(20 rabbits,subconjunctivally injected with 0.1 mL of 1×PBS every two days)and the intervention group(20 rabbits,subconjunctivally injected with 0.5 mg·L^(-1)Scg3 antibody every two days).On the 14th day after alkali burn,the protein expression of Scg3 in the corneal tissue of rabbits in the control group and the model group was detected with Western blot,respectively.On the 1st,7th and 14th day after alkali burn,the area and length of CNV were measured by the anterior segment analyzer,the ocular surface changes were observed under the corneal confocal microscope,and the expression levels of inflammation-associated cytokines such as intercellular adhesion molecule-1(ICAM-1),interleukin-10(IL^(-1)0),and transforming growth factor-β1(TGF-β1)were evaluated by the protein array method.Results On the 14th day after alkali burn,there was significant CNV growth in the model group but not in the intervention group.Western blot results showed that compared with the control group,the Scg3 protein expression in the model group was significantly elevated(P<0.05).Confocal microscopy also showed substantial active CNV in the corneal limbus of model rabbits.On the 7th and 14th day after alkali burn,the length and area of CNV in the intervention group were significantly lower than those in the model group(both P<0.001).Similarly,on the 7th and 14th day after alkali burn,the expression levels of ICAM-1,IL^(-1)0 and TGF-β1 in the intervention group were significantly lower than those in the model group(all P<0.05).Conclusion The 0.5 mg·L^(-1)Scg3 antibody can reduce the inflammatory response and inhibit the CNV growth in rabbits af

关 键 词：角膜新生血管 分泌粒蛋白Ⅲ 血管生长因子 角膜碱烧伤 

分 类 号：R772.2[医药卫生—眼科]