EPAS1 siRNA抑制人肾透明细胞癌细胞系HiMet⁃ccRCC的增殖、迁移及侵袭  

EPAS1 siRNA inhibits the proliferation,migration and invasion of human renal clear cell carcinoma cell line HiMet⁃ccRCC

作　　者：陈海滨[1] 赵建军[1] 谭超 CHEN Hai-bin;ZHAO Jian-jun;TAN Chao(Department of Urology,the Affiliated Hospital of Hebei University of Engineering,Handan 056004,China)

机构地区：[1]河北工程大学附属医院泌尿外二科,河北邯郸056004

出　　处：《基础医学与临床》2022年第10期1522-1527,共6页Basic and Clinical Medicine

基　　金：河北省卫生健康委员会基金(20200591)。

摘　　要：目的探讨沉默内皮PAS区域蛋白1(EPAS1)对抑制人肾透明细胞癌细胞系HiMet⁃ccRCC增殖、迁移和侵袭的影响及可能机制。方法将HiMet⁃ccRCC细胞分为对照(Con)组、阴性对照(NC)组、沉默EPAS1表达组(EPAS1 siRNA组)。用RT⁃qPCR检测HiMet⁃ccRCC细胞EPAS1 mRNA水平;MTT法检测HiMet⁃ccRCC细胞增殖;Transwell小室法检测HiMet⁃ccRCC细胞迁移、侵袭能力;蛋白质印迹法检测E⁃cadherin、N⁃cadherin、MMP⁃9、PCNA表达。结果与对照组、阴性对照组比较,EPAS1 siRNA组HiMet⁃ccRCC细胞中EPAS1表达水平显著降低(P<0.05),HiMet⁃ccRCC细胞增殖抑制率明显升高、侵袭及迁移细胞数明显减少(P<0.05);与对照组、阴性对照组比较,EPAS1 siRNA组HiMet⁃ccRCC细胞E⁃cadherin蛋白表达上调(P<0.05),N⁃cadherin、MMP⁃9、PCNA蛋白表达下调(P<0.05)。结论沉默EPAS1可抑制HiMet⁃ccRCC细胞增殖、迁移及侵袭,EPAS1可能是肾透明细胞癌基因治疗的潜在生物靶标。Objective To explore the effects of silencing endothelial PAS domain⁃containing protein 1(EPAS1)on the proliferation,migration and invasion of human renal clear cell carcinoma cell line HiMet⁃ccRCC and to discuss the possible mechanism.Methods HiMet⁃ccRCC cells were divided into control(Con)group,negative control(NC)group and silencing EPAS1 expression group(EPAS1 siRNA group).RT⁃qPCR was used to detect the EPAS1 mRNA level in HiMet⁃ccRCC cells;MTT method was used to detect the proliferation of HiMet⁃ccRCC cells;Transwell experiment was used to detect the migration and invasion ability of HiMet⁃ccRCC cells;Western blot was used to detect the expression of E⁃cadherin,N⁃cadherin,MMP⁃9,PCNA.Results Compared with Con and NC group,the expression level of EPAS1 in HiMet⁃ccRCC cells in the EPAS1 siRNA group was significantly reduced(P<0.05),the proliferation inhibition rate of HiMet⁃ccRCC cells was significantly increased and the counting of invasion and migration cells was significantly reduced(P<0.05),the protein expression of E⁃cadher⁃in in HiMet⁃ccRCC cells from EPAS1 siRNA group increased(P<0.05)and the protein expression of N⁃cadher⁃in,MMP⁃9 and PCNA reduced(P<0.05).Conclusions Silencing EPAS1 could inhibit the proliferation,mi⁃gration and invasion of HiMet⁃ccRCC cells and EPAS1 may be a potential biological target for gene therapy of re⁃nal clear cell carcinoma.

关 键 词：内皮PAS区域蛋白1 肾透明细胞癌 增殖 迁移 侵袭 

分 类 号：R737.11[医药卫生—肿瘤]