一株人轮状病毒G2P[4]型毒株的近似全基因组进化分析  

Approximate genome-wide evolutionary analysis of one human rotavirus G2P[4]strain

作　　者：高升辉 李利利[2] 李丹地[2] 朱曦 王萌璇 王明雯 吴建军 李金松[2] Gao Shenghui;Li Lili;Li Dandi;Zhu Xi;Wang Mengxuan;Wang Mingwen;Wu Jianjun;Li Jinsong(School of Public Health,Gansu University of Traditional Chinese Medicine,Lanzhou 730099,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]甘肃中医药大学公共卫生学院,兰州730099 [2]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华实验和临床病毒学杂志》2022年第4期402-408,共7页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重点研发计划"病原学与防疫技术体系研究"专项"难培养和微量病原体靶向培养技术研究"项目(2021YFC2301000);国家科技重大专项工作推进路线图"基于海量多元大数据的突发急性传染病时空多尺度预测预警模型与应用示范的构建"(2018ZX10201002)。

摘　　要：目的分析轮状病毒G2P[4]型2020BJ株的近似全基因组进化特征。方法运用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)进行轮状病毒基因组扩增,将扩增产物测序,对所得序列进行系统进化和同源性分析。结果获得人轮状病毒G2P[4]型2020BJ株近似全长的11个节段核酸序列,序列分析表明其为G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2基因型(DS-1-Like);进化分析表明与日本、印度、孟加拉及意大利等国家毒株亲缘关系较近;亲缘关系较近的毒株间抗原表位的氨基酸存在差异。结论与2020BJ亲缘关系较近的5株G2P[4]型轮状病毒VP7和VP4的抗原表位的氨基酸存在差异,可能导致流行特征不同,应加强轮状病毒监测。Objective To analyze the evolutionary characteristics of the approximate whole genome of rotavirus G2P[4]type 2020BJ strain.Methods The rotavirus genome was amplified by reverse transcription-polymerase chain reaction(RT-PCR),the amplified products were sequenced,and the sequences were subjected to phylogenetic analysis and homology analysis.Results The approximate full-length 11 segments of human rotavirus G2P[4]type 2020BJ strain were obtained.Sequence analysis showed that the strain was G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotype(DS-1-Like);evolutionary analysis shows that it is closely related to strains in Japan,India,Bangladesh,Italy and other countries;there are differences in the amino acids of antigenic epitopes between the closely related strains.Conclusions There are differences in the amino acids of the epitopes of VP7 and VP4 of the five G2P[4]rotavirus strains that are closely related to 2020BJ,which may lead to different epidemic characteristics,and rotavirus surveillance should be strengthened.

关 键 词：轮状病毒 基因型 全基因组 系统进化树 

分 类 号：R373.2[医药卫生—病原生物学]