C30色谱填料分离高纯辅酶Q10的尝试  

Separation of High Purity Coenzyme Q10 by Pre-HPLC with C30

作　　者：王洪宇 张慧君 WANG Hong-yu;ZHANG Hui-jun(Tianjin Zhongke Biosepur Biotechnology Co.,LTD,Tianjin 300300,China)

机构地区：[1]天津中科博蕴生物技术有限公司,天津300300

出　　处：《海峡药学》2022年第8期74-76,共3页Strait Pharmaceutical Journal

摘　　要：目的建立一种安全、节能的制备液相色谱分离辅酶Q10的方法。方法采用C30烷基键合硅胶为固定相的高压制备液相色谱,以丙酮/甲醇为流动相,分离去除辅酶Q10中的杂质Q11,上样量达到了固定相量的5.8%;样品回收率达到了87.3%。结果该工艺在28~32℃的条件下进行,节省了大量用于流动相加热的能量,降低了加热带来的系统风险,节约了由加热系统带来的成本,节省了设备空间。结论该工艺可以作为类似辅酶Q10类发酵产物量产的高纯物分离的一种新尝试。OBJECTIVE To establish a safe and energy-saving method for the separation of coenzyme Q10 by liquid chromatography.METHODS Attempt to separate and remove impurity Q11 in coenzyme Q10 with pre-HPLC by C30 bonded silica gel as stationary phase and acetone/methanol as mobile phase.The loading amount was 5.8%of the weight of stationary phase;The recovery rate of the sample was 87.3%.RESULTS The process was carried out at 28-32℃,which saved a lot of energy for heated mobile phase,reduced the system risk caused by heating and saved the cost caused by the heating system and saved equipment space.CONCLUSION From the perspective of safety and energy saving,it can be used as a new attempt for the separation of high-purity products similar to the mass production of coenzyme Q10 fermentation products.

关 键 词：C30 高压制备色谱 辅酶Q10 Q11 

分 类 号：R927[医药卫生—药学]