碱性蛋白酶水解羊血清蛋白的工艺研究  

Study on optimum conditions of hydrolyzation of sheep serum protein by alkaline protease

作　　者：李昕宇 马金璞 张玉璇 郭星晨 杨具田[1,2] 徐红伟[1,2] 曹忻 高丹丹[1,2] Li Xinyu;Ma Jinpu;Zhang Yuxuan;Guo Xingchen;Yang Jutian;Xu Hongwei;Cao Xin;Gao Dandan(College of Life Science and Engineering,Northwest Minzu University,Gansu Lanzhou 730124;Biomedical Research Center,Northwest Minzu University,Gansu Lanzhou 730030)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124 [2]西北民族大学生物医学研究中心,甘肃兰州730030

出　　处：《现代畜牧兽医》2022年第8期26-30,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：西北民族大学中央高校基本科研业务费资金资助项(31920220026);西北民族大学大学生创新创业训练计划项目(X202210742306);甘肃省教育厅青年博士基金项目(2021QB-147)。

摘　　要：试验旨在探究羊血清蛋白的水解的最佳工艺条件,以羊血为原料,考察酶解温度、pH值、水解时间和加酶量等4个单因素对羊血清蛋白水解的影响程度,采用响应面分析法优化其水解工艺参数。结果显示,最佳工艺条件为:温度51.2℃、水解pH值11.59、水解时间5.10 h、酶添加量为3155 U/g;在此水解条件下,羊血清蛋白水解度为26.97%。The purpose of the experiment was to explore the optimal process conditions for the hydrolysis of goat serum protein.The experiment used sheep blood as raw material to investigate the influence of four factors including enzymatic hydrolysis temperature,pH value,hydrolysis time and amount of enzyme added on the proteolysis of sheep serum,and optimized the hydrolysis process parameters by response surface methodology.The results showed that the optimum process conditions were temperature 51.2℃,hydrolysis pH value 11.59,hydrolysis time 5.10 h and enzyme addition amount 3155 U/g.Under the hydrolysis condition,the degree of hydrolysis of goat serum protein was 26.97%.

关 键 词：羊血清蛋白质 碱性蛋白酶 响应面 水解度 

分 类 号：S872[农业科学—畜牧兽医]