柴胡皂苷d对大鼠肝星状细胞TGFβ-Smad信号通路mRNA表达的影响  

Influence of Saikosaponins-d on the Expressions of TGFβ-Smad Signal Path mRNA in Rat Hepatic Stellate Cells

作　　者：陈懿榕 阙任烨 刘进锴[3] 林柳兵[1] 李勇[1] CHEN Yirong;QUE Renye;LIU Jinkai;LIN Liubing;LI Yong(Shanghai City Hospital of Traditional Chinese Medicine,Shanghai 200041,China;Shanghai TCM-integrated Hospital,Shanghai University of TCM;Eastern Hepatobiliary Surgery Hospital affiliated to the Second Military Medical University)

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海中医药大学附属上海市中西医结合医院 [3]第二军医大学附属东方肝胆外科医院

出　　处：《西部中医药》2022年第8期15-19,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81573775,81873157);上海市科委引导类项目(18401904000);上海市青年科技英才扬帆计划(19YF1445200);上海市卫健委中发办青年基金(2018LQ016);上海中医药大学预算内项目(2021LK067)。

摘　　要：目的:观察柴胡皂苷d(saikosaponins-d,SSd)对肝星状细胞TGFβ_(1)、Smad3/7mRNA表达的影响。方法:采用大鼠肝星状细胞(hepatic stellate cell-T6,HSC-T6)为模型,采用药物进行干预后加入H_(2)O_(2)诱导氧化应激,采用RT-PCR实验方法检测TGFβ_(1)、Smad3/7 mRNA表达。结果:与空白组比较,模型组TGFβ_(1)、Smad3表达增强,Smad7表达减弱(P<0.05)。与模型组比较,SSd组干预后HSC-T6细胞中TGFβ_(1)、Smad3 mRNA水平降低,Smad7 mRNA水平增高(P<0.05)。这种作用可被雌激素受体(estrogen receptor,ER)完全拮抗剂和ERβ拮抗剂阻滞。结论:SSd能下凋TGFβ_(1)、Smad3 mRNA表达,上调Smad7 mRNA的表达,对HSC-T6的TGFβ-Smad信号通路有明显的影响,可能是SSd对肝纤维化产生治疗作用的机制之一。Objective:To observe the effects of SSd on the expressions of TGFβ_(1),Smad3/7mRNA in hepatic stellate cells.Methods:Rat HSC-T6 were adopted as the model,after drug intervention,H_(2)O_(2) was added to induce oxidative stress,RT-PCR was used to detect the expressions of TGFβ_(1),Smad3/7mRNA.Results:Compared with the blank group,the expression of TGFβ_(1)and Smad3 in the model group was enhanced,while the expression of Smad7 was weakened(P<0.05).Compared with the model group,the levels of TGFβ_(1)and Smad3 mRNA were decreased while the level of Smad7mRNA was elevated in HSC-T6 cells in SSd group after the intervention(P<0.05).The effects could be blocked by ER complete antagonist and ERβantagonist.Conclusion:SSd could down regulate the expressions of TGFβ_(1),Smad3 mRNA,up regulate the expressions of Smad7 mRNA,and it could affect TGFβ-Smad signal pathway in HSC-T6 signal pathway,which might be one of the mechanisms of SSd producing therapeutic effects on liver fibrosis.

关 键 词：肝纤维化 柴胡皂苷D 雌二醇 肝星状细胞 TGFβ-Smad信号通路 动物实验 大鼠 

分 类 号：R575.2[医药卫生—消化系统]