藻酸盐对海假交替单胞菌生物被膜形成及厚壳贻贝附着变态的影响  

Effects of alginate on biofilm formation of Pseudoalteromonas marina and larval settlement and metamorphosis of the mussel Mytilus coruscus

作　　者：陈慧娥 贺康 贺楚晗 竹攸汀[1,2] 杨金龙 梁箫[1,2] CHEN Huie;HE Kang;HE Chuhan;ZHU Youting;YANG Jinlong;LIANG Xiao(International Research Center for Marine Biosciences,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals,Shanghai 201306,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China)

机构地区：[1]上海海洋大学国家海洋生物科学国际联合研究中心,上海201306 [2]上海市水产动物良种创制与绿色养殖协同创新中心,上海201306 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458

出　　处：《大连海洋大学学报》2022年第4期620-626,共7页Journal of Dalian Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”项目(2020YFD0900804);国家自然科学基金(41876159,41476131);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402);上海市优秀学术带头人计划项目(20XD1421800);枸杞岛海域贻贝养殖的种质、环境及养殖策略研究课题。

摘　　要：为探究藻酸盐对海假交替单胞菌Pseudoalteromonas marina生物被膜形成及厚壳贻贝Mytilus coruscus幼虫附着变态的影响,以海假交替单胞菌生物被膜为对照组,将终质量浓度为0.1、0.5、1.0、1.5、2.0 mg/L的藻酸盐标准品溶液,分别添加到海假交替单胞菌菌液中共同形成生物被膜,检测生物被膜对厚壳贻贝幼虫附着变态的影响,并分析了藻酸盐对生物被膜的膜厚及胞外产物的影响。结果表明:生物被膜的细菌密度随添加藻酸盐质量浓度的增加而增加;当添加藻酸盐质量浓度为0.5 mg/L时,共同形成的生物被膜的膜厚和胞外多糖均显著增加(P<0.05),诱导厚壳贻贝幼虫附着变态的能力增强。研究表明,添加藻酸盐能够促进P.marina生物被膜的形成,且可拉酸等其他胞外多糖含量的增加促进了生物被膜对厚壳贻贝幼虫附着变态的诱导能力,藻酸盐的适宜添加量为0.5 mg/L。In order to research effects of alginate on biofilm formation of bacterium Pseudoalteromonas marina and the roles of co-formed biofilm on larval settlement and metamorphosis of larval mussel Mytilus coruscus,the settlement and metamorphosis of the mussel larvae were investigated on biofilm of P.marina as a control,and the bacterial biofilm containing alginate solution at final concentration of 0.1,0.5,1.0,1.5 and 2.0 mg/L.In addition,the optimal concentration of alginate was selected to compare and analyze the changes in density,thickness and extracellular products of the biofilm.The bacterial density on the biofilms was shown to be increased with the increase in the alginate concentration.There were significantly increase in thickness and extracellular polysaccharide contents of the biofilm at alginate concentration of 0.5 mg/L(P<0.05),and finally increase in biofilm-inducing activities.The findings have positive significance for understanding of the induction mechanism of marine shellfish larval settlement and metamorphosis,and provide theoretical basis for the use of biofilms to improve the success rate of mussel seedlings.The appropriate concentration of alginate is 0.5 mg/L.

关 键 词：厚壳贻贝 藻酸盐 生物被膜 幼虫附着变态 

分 类 号：S968.3[农业科学—水产养殖]