延胡索总碱贴片经神阙穴与非穴位给药的皮肤渗透性比较  

Comparison on Skin Permeability of Corydalis Rhizoma Total Alkaloid Patches at Shenque Acupoint and Non-acupoint Administration

作　　者：姜明瑞 王志成 岳珠珠 张婧秋 王佩华 张桂梅 王慧楠 杨子烨 陈梦雨 王英姿[1] JIANG Mingrui;WANG Zhicheng;YUE Zhuzhu;ZHANG Jingqiu;WANG Peihua;ZHANG Guimei;WANG Huinan;YANG Ziye;CHEN Mengyu;WANG Yingzi(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中国实验方剂学杂志》2022年第19期148-153,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2018YFE0197900)。

摘　　要：目的:比较穴位与非穴位皮肤的电阻与皮肤结构差异,并研究延胡索总碱贴片经神阙穴和非穴位给药后的皮肤渗透特性,为其临床穴位贴敷治疗慢性疼痛提供实验支持。方法:以延胡索甲素、延胡索乙素、延胡索丑素为评价指标,结合高效液相色谱法(HPLC)进行定量分析,色谱条件为流动相甲醇-0.04 mol·L^(-1)磷酸水溶液(70∶30,用三乙胺调pH 6.0),检测波长281 nm。采用Franz扩散池离体透皮试验与大鼠在体透皮试验相结合的方式,研究延胡索总碱贴片经神阙穴与非穴位给药后的皮肤渗透特性。同时,测定给药前后神阙穴与非穴位皮肤电阻,采用冷冻切片法比较药物在皮肤各层中的分布情况,并运用荧光倒置显微镜进行可视化验证。结果:给药24 h后,体内外试验结果均显示,延胡索总碱贴片经神阙穴贴敷时延胡索甲素、延胡索乙素和延胡索丑素的累积透过量与滞留量均明显高于非穴位皮肤(P<0.05,P<0.01),神阙穴皮肤电阻在给药前后各时间点均低于非穴位皮肤。荧光显微观察结果显示,皮肤各层的药物含量均为神阙穴皮肤>非穴位皮肤,表明神阙穴皮肤对于药物的渗透、贮存作用优于非穴位皮肤。结论:延胡索总碱贴片在神阙穴皮肤的24 h累积透过量和滞留量均高于非穴位皮肤,其机制可能与神阙穴皮肤薄、电阻低及毛囊小体数量多有关。Objective:To compare the differences in resistance and structure of skin between acupoints and non-acupoints,and to study the difference in skin permeability characteristics of Corydalis Rhizoma total alkaloid patches(CTTP)after administration at Shenque acupoint and non-acupoint,so as to provide experimental support for its clinical acupoint application to prevent and treat chronic pain.Method:Taking corydaline(CD),tetrahydropalmatine(THP)and corydalis L(CDL)as evaluation indexes,and the quantitative analysis was carried out by high performance liquid chromatography(HPLC).The mobile phase was methanol-0.04 mol·L^(-1)phosphoric acid aqueous solution(70∶30,pH 6.0 adjusted with triethylamine),the detection wavelength was 281 nm.In vitro transdermal test in Franz diffusion cell and in vivo transdermal test were used to study the skin permeability characteristics of CTTP through Shenque acupoint and non-acupoint administration.At the same time,the skin resistance between Shenque acupoint and non-acupoint was measured before and after the administration,and the distribution of the drug in each layer of the skin was compared by freezing sectioning,and visual verification was performed with fluorescence inverted microscope.Result:After 24 h of administration,the results of in vivo and in vitro experiments showed that the cumulative permeation and retention of CD,THP and CDL at Shenque acupoint skin were higher than those at non-acupoint skin(P<0.05,P<0.01),the skin resistance of Shenque acupoint was lower than that of non-acupoint at all time points.The fluorescence microscopic observation results showed that the drug content of each layer of the skin was all Shenque acupoint>non-acupoint,indicating that the skin of Shenque acupoint had better effect on drug penetration and storage than non-acupoint.Conclusion:The 24 h cumulative permeation and retention of CTTP in Shenque acupoint skin are higher than those in non-acupoint skin,and the mechanism may be related to the thin skin,low electrical resistance and large num

关 键 词：延胡索总碱贴片 穴位贴敷 神阙穴 皮肤渗透性 高效液相色谱法(HPLC) 皮肤电阻 FRANZ扩散池 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学] R94R96