miR-29家族通过AKT/mTOR信号通路抑制结肠癌MC38细胞的增殖、迁移和侵袭  

miR-29 Family Inhibits the Proliferation,Migration and Invasion of Colon Cancer MC38 Cells Through the AKT/mTOR Signaling Pathway

作　　者：郑诚 东方阳 程蕾 刘可可 毋文静 张瑾[2] ZHENG Cheng;DONGFANG Yang;CHENG Lei;LIU Keke;WU Wenjing;ZHANG Jin(College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Qinhuangdao 066000,China;College of Biological Chemical Sciences and Engineering,Jiaxing University,Jiaxing 314000,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,秦皇岛066000 [2]嘉兴学院生物与化学工程学院,嘉兴314000

出　　处：《中国细胞生物学学报》2022年第5期798-806,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:32102506、32172708);嘉兴市公益性研究计划(批准号:2021AY10046);浙江省医学电子与数字健康重点实验室和生命健康智能感知浙江省工程研究中心资助的课题。

摘　　要：结肠癌是最常见的恶性肿瘤之一,死亡人数仅次于肺癌。有研究发现miR-29家族成员(miR-29a、miR-29b和miR-29c)在结肠癌组织中的表达水平显著低于癌旁组织,但其对结肠癌的影响不明。为探讨miR-29家族对结肠癌的影响及作用机制,该研究在结肠癌细胞系MC38中分别转染miR-29a/b/c-3p mimics,利用qRT-PCR、CCK-8、EdU染色等实验确定miR-29家族成员可以显著降低MC38细胞的增殖能力(P<0.05);通过划痕实验和Transwell侵袭实验,明确miR-29家族成员可显著抑制MC38细胞的迁移和侵袭(P<0.05);最后利用信号通路活性分析、双荧光素酶报告分析等确定miR-29家族成员能够抑制AKT/mTOR信号通路的活性,并且与束蛋白结合蛋白1(Fascin actin-bundling protein 1,FSCN1)存在相互作用。上述结果表明,miR-29家族成员能够抑制AKT/mTOR信号通路活性,进而降低结肠癌细胞MC38增殖、迁移和侵袭的能力。Colon cancer is one of the most common malignant tumors.The number of deaths caused by colon cancer is second only to lung cancer.Studies have found that the expression of the miR-29 family members (miR-29a,miR-29b,and miR-29c) in colon cancer tissue was significantly lower than in paracancerous tissue,but its effect on colon cancer is unknown.In order to explore the effect and mechanism of the miR-29 family members on colon cancer,a colon cancer cell line MC38 was used for the transfection of miR-29a/b/c-3p mimics individually in this research.qRT-PCR,CCK-8 and EdU staining experiments proved that the miR-29 family members could inhibit the proliferation of MC38 cells (P<0.05).Cell wound scratch assay and Transwell invasion experiment showed that the miR-29 family members could suppress the migration and invasion of MC38 cells (P<0.05).Finally,the signaling pathway analysis and dual-luciferase report assay indicated that the miR-29 family members could decrease the activity of AKT/mTOR signaling pathway and interact with FSCN1 (Fascin actin-bundling protein 1).The above results indicated that the miR-29 family members could reduce the proliferation,migration and invasion of colon cancer cell line MC38 by inhibiting the activity of AKT/mTOR signaling pathway.

关 键 词：MIR-29 结肠癌 增殖 迁移 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]