过表达类光捕获蛋白3降低水稻籽粒中三烯生育酚含量  

Overexpression of LIL3 reduces tocotrienol contents in rice grains

作　　者：赖晓凤 罗凯晴 万小荣[1] 陈洁[1] 郎兴宣 马晓智 李春梅 LAI Xiaofeng;LUO Kaiqing;WAN Xiaorong;CHEN Jie;LANG Xingxuan;MA Xiaozhi;LI Chunmei(Guangzhou Key Laboratory of Research and Development of Crop Germplasm Resources,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]仲恺农业工程学院广州市特色作物种质资源研究与利用重点实验室,广东广州510225 [2]广东省农业科学院水稻研究所,广东广州510640

出　　处：《仲恺农业工程学院学报》2022年第3期28-33,共6页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广州市特色作物种质资源研究与利用重点实验室项目(202002010010);广东省基础与应用基础研究粤穗联合青年基金项目(2019A1515110867);国家自然科学基金(31770652、 32071737和32111530289);广东省教育厅重点领域项目资助;仲恺农业工程学院研究生科技创新基金项目(KJCX2021011)资助。

摘　　要：类光捕获蛋白3(Light-harvesting like protein 3,LIL3)是光捕获复合物蛋白超家族(Light-harvesting complex,LHC)的一员,其特征是存在一个保守的LHC motif序列.水稻(Oryza sativa L.)LIL3参与叶绿素、生育酚侧链的生物合成.维生素E包括生育酚和三烯生育酚2种,是一类重要的脂溶性化合物,具有抗氧化活性.为了探究LIL3对水稻籽粒维生素E含量的影响,在日本晴(Oryza Sativa spp.Japonica.)中过表达LIL3,得到了稳定的转基因株系.分别对野生型和过表达植株穗长、每穗粒数、粒长、粒宽以及籽粒中的维生素E含量做了分析.结果表明:与野生型相比,LIL3过表达植株的穗长、粒长和粒宽没有明显变化,每穗籽粒数略有所下降.籽粒中三烯生育酚含量降低.Light-harvesting like protein 3(LIL3) is a member of the light-harvesting complex family(LHC), which is characterized by a conserved LHC motif sequence. Rice(Oryza sativa L.) LIL3 participates in the side chain biosynthesis of chlorophyll and tocopherol. Vitamin E, including tocopherols and tocotrienols, is an important fat-soluble compound with antioxidant activity. In order to explore the effects of LIL3 on vitamin E contents in rice grains, LIL3 was overexpressed in Nipponbare(Oryza Sativa spp. Japonica.), and a stable transgenic line was obtained. The panicle length, number of grains per panicle, grain length, grain width and the content of vitamin E in grains were analyzed, respectively, in wild-type and overexpressing plants. The results showed that the panicle length, grain length and width of LIL3 overexpression plants did not change significantly, compared with those of the wild type. However, number of grains per panicle decreased slightly. In addition, the total contents of tocotrienols obviously decreased in the LIL3 overexpressing plants.

关 键 词：水稻 维生素E 类光捕获蛋白LIL3 

分 类 号：S565[农业科学—作物学]