两种黄酮对HepG-2肝癌细胞增殖抑制作用的对比研究  

Comparative study on the inhibitory effects of two flavonoids on the proliferation of HepG-2 hepatoma cells

作　　者：刘建华[1] 孔丽宇 万建伟 苏倩榕 李景华[1] 韩丽琴[1] LIU Jianhua;KONG Liyu;WAN Jianwei;SU Qianrong;LI Jinghua;HAN Liqin(Jilin Medical University,Jilin City,Jilin Province,133013,China)

机构地区：[1]吉林医药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2022年第5期346-348,共3页Journal of Jilin Medical University

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20200466 KJ);吉林省卫健委项目(20192c006);吉林省大学生创新创业项目(201913706048);吉林省大学生创新创业项目(202113706074).

摘　　要：目的对比黄芪黄酮和刺五加黄酮对HepG-2肝癌细胞抑制率的差异。方法闪式提取法提取样品中总黄酮,流式细胞术检测黄芪黄酮和刺五加黄酮对HepG-2肝癌细胞增殖的抑制率。结果当黄芪黄酮浓度达到100μmol/L时,细胞已经大量脱壁死亡;流式细胞术检测结果显示,两种黄酮化合物对于肝癌细胞增殖有很好的抑制作用,且抑制率与药物浓度间有剂量-效应关系。结论黄芪黄酮对HepG-2肝癌细胞抑制率远高于刺五加黄酮。Objective To compare the inhibitory rates of astragalus flavonoids and acanthopanax senticosus flavonoids on HepG-2 hepatoma cells.Methods The total flavonoids were extracted by flash extraction,and the inhibitory rates of astragalus flavonoids and acanthopanax senticosus flavonoids on the proliferation of HepG-2 hepatoma cells were detected by flow cytometry.Results When the concentration of astragalus flavonoids reached 100μmol/L,a large number of cells have fallen off the wall and died;The results of flow cytometry showed that the two flavonoids had a good inhibitory effect on the proliferation of hepatoma cells,and there was a dose-response relationship between the inhibitory rate and the drug concentration.Conclusion The inhibitory rate of astragalus flavonoids on HepG-2 hepatoma cells is much higher than that of acanthopanax senticosus flavonoids.

关 键 词：黄芪 刺五加 黄酮 HEPG-2 流式细胞术 

分 类 号：R914.22[医药卫生—药物化学]