早期胃癌患者外周血CDH-17,RHBDD1和miR-135表达水平与临床病理特征的相关性研究  

Correlation between the Expression Levels of CDH-17,RHBDD1 and miR-135 in Peripheral Blood and Clinicopathological Features in Patients with Early Gastric Cancer

作　　者：王燕云[1] 初丽敏 王婵 胡美红[1] 杨晓 贺丽珠 WANG Yan-yun;CHU Li-min;WANG Chan;HU Mei-hong;YANG Xiao;HE Li-zhu(Department of Gastroenterology,the Third Hospital of Shijiazhuang City,Shijiazhuang 050000,China;Department of Geriatrics,the Third Hospital of Shijiazhuang City,Shijiazhuang 050000,China)

机构地区：[1]石家庄市第三医院消化内科,石家庄050000 [2]石家庄市第三医院老年病科,石家庄050000

出　　处：《现代检验医学杂志》2022年第5期18-22,共5页Journal of Modern Laboratory Medicine

基　　金：石家庄市科学技术研究与发展计划项目,编号:211461103。

摘　　要：目的 探究早期胃癌患者外周血肝肠-钙黏连蛋白-17(liver-intestine cadherin-17,CDH-17)、菱形结构域蛋白1(rhombic domain-containing protein 1,RHBDD1)和微小核糖核酸(micro RNA,miR)-135表达水平与临床病理特征的相关性。方法 回顾性选取2018年5月~2021年6月石家庄市第三医院105例早期胃癌患者作为观察组,另选取同期健康体检者60例作为对照组,以酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定CDH-17和RHBDD1水平,实时荧光聚合酶链反应(real-time fluorescence PCR,RT-PCR)测定miR-135表达,比较两组外周血CDH-17,RHBDD1和miR-135表达,分析外周血CDH-17,RHBDD1和miR-135与早期胃癌及临床病理特征的关系,绘制受试者工作特征(receiver operating characteristic,ROC)曲线,评价外周血CDH-17,RHBDD1和miR-135对早期胃癌的诊断价值。结果 与对照组相比,观察组外周血CDH-17(72.41±20.14 pg/ml vs59.69±14.37pg/ml),RHBDD1(374.26±115.74 ng/ml vs 214.37±56.41ng/ml)和miR-135(3.87±1.26 vs 2.47±0.81)表达均升高,差异有统计学意义(t=4.304,10.032,7.736,均P<0.05);Logistic回归分析,结果显示外周血CDH-17[OR=11.497,95%CI=7.415~17.825],RHBDD1[OR=13.748,95%CI=9.827~19.234]和miR-135[OR=11.654,95%CI=7.946~17.991]表达为早期胃癌独立危险因素(Wald χ^(2)=38.417,33.771,37.877,均P<0.05)。外周血CDH-17,RHBDD1和miR-135表达对早期胃癌的诊断价值的ROC曲线显示外周血CDH-17,RHBDD1和miR-135表达对早期胃癌的诊断价值的AUC分别为0.736,0.903和0.828。应用Logistic二元回归拟合,构建各指标联合诊断的AUC为0.947;外周血CDH-17,RHBDD1和miR-135表达与临床分期、组织学分级有关(r=0.625,0.631,0.657;-0.633,-0.625,-0.674,均P<0.05)。结论 早期胃癌患者外周血CDH-17,RHBDD1和miR-135表达明显上调,且与临床分期及组织学分级有关。临床可通过检测其水平,准确诊断早期胃癌,为临床治疗提供一定依据,对改善患者预后具有重要意义。Objective To explore the correlation between the levels of liver-intestine cadherin-17(CDH-17),rhombic domain-containing protein 1(RHBDD1)and miR-135 in peripheral blood and clinicopathological features in patients with early gastric cancer.Methods From May 2018 to June 2021,105 patients with early gastric cancer in the Third Hospital of Shijiazhuang City,were retrospectively selected as the observation group,and 60 patients with healthy physical examination during the same period were selected as the control group.To compare the expressions of CDH-17,RHBDD1 and miR-135 in peripheral blood of the two groups,analyzed the relationship between CDH-17,RHBDD1and miR-135 in peripheral blood and early gastric cancer and clinicopathological characteristics.Drew receiver operating characteristic(ROC)curve,and evaluated peripheral the diagnostic value of blood CDH-17,RHBDD1 and miR-135 in early gastric cancer.Results In the observation group,peripheral blood CDH-17(72.41±20.14pg/ml vs 59.69±14.37pg/ml),RHBDD1(374.26±115.74ng/ml vs 214.37±56.41ng/ml),miR-135(3.87±1.26 vs 2.47±0.81)expression was higher than the control group,the differences were statistically significant(t=4.304,10.032,7.736,all P<0.05).Logistic regression analysis showed that the expression of CDH-17[OR=11.497,95%CI=7.415~17.825],RHBDD1[OR=13.748,95%CI=9.827~19.234] and miR-135[OR=11.654,95%CI=7.946~17.991] in peripheral blood were independent risk factors for early gastric cancer(Wald χ^(2)=38.417,33.771,37.877,all P<0.05).The ROC curve of the diagnostic value of peripheral blood CDH-17,RHBDD1 and miR-135 expression for early gastric cancer showed that the AUC of the peripheral blood CDH-17,RHBDD1 and miR-135 expression for the diagnostic value of early gastric cancer was 0.736,0.903 and 0.828,respectively.Logistic binary regression fitting to construct the AUC of each index combined diagnosis showed that the combined AUC of each index was 0.947.The expression of CDH-17,RHBDD1 and miR-135 in peripheral blood was related to clinical stage and histol

关 键 词：早期胃癌 临床分期 组织学分级 肝肠-钙黏连蛋白-17 菱形结构域蛋白1 微小核糖核酸-135 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]