肿瘤坏死因子-β基因多态性与大动脉粥样硬化型脑梗死的相关性  

Association between tumor necrosis factor-βgene polymorphisms and large artery atherosclerotic cerebral infarction

作　　者：刘利宁[1] 李毓新[1] 王晓芳 LIU Lining;LI Yuxin;WANG Xiaofang(Department of Neurology,The Second Affiliated Hospital of Xi′an Medical College,Xi′an,Shaanxi 710038,China)

机构地区：[1]西安医学院第二附属医院神经内科,陕西西安710038

出　　处：《检验医学与临床》2022年第19期2596-2600,共5页Laboratory Medicine and Clinic

基　　金：西安医学院校级科研计划项目(12FZ06)。

摘　　要：目的探讨肿瘤坏死因子-β(TNF-β)A252G、C804A基因多态性与大动脉粥样硬化型(LAA)脑梗死的相关性。方法选取该院收治的80例LAA脑梗死患者作为脑梗死组,选取同一时期门诊体检健康者80例作为对照组,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术和聚合酶链反应-序列特异性引物法(PCR-SSP)进行TNF-βA252G、C804A基因分型检测,并收集临床资料。同时,检测空腹血糖(FBG)、总胆固醇、三酰甘油、低密度脂蛋白胆固醇、血同型半胱氨酸。结果脑梗死组中合并高血压、糖尿病、吸烟史患者所占比例及FBG水平均显著高于对照组(P<0.05)。脑梗死组TNF-βA252G基因型分布与对照组相比,差异有统计学意义(P<0.05),其中GG基因型频率、G等位基因频率均显著高于对照组(P<0.05)。脑梗死组TNF-βC804A基因型分布与对照组相比,差异有统计学意义(P<0.05),其中AA基因型频率、A等位基因频率均显著高于对照组(P<0.05)。多因素Logistic回归分析显示高血压、糖尿病、吸烟史、FBG水平、TNF-βA252G基因型为GG型、TNF-βA252G等位基因为G型、TNF-βC804A基因型为AA型、TNF-βC804A等位基因为A型均为LAA脑梗死发生的独立危险因素(P<0.05)。结论TNF-βA252G基因型为GG型、TNF-βA252G等位基因为G型、TNF-βC804A基因型为AA型、TNF-βC804A等位基因为A型可能是LAA脑梗死发生的重要易感基因。Objective To determine the association between tumor necrosis factor-β(TNF-β)A252G and C804A gene polymorphisms and large artery atherosclerotic(LAA)cerebral infarction(CI).Methods A total of 80 cases of LAA CI patients admitted to our hospital were enrolled into CI group,and 80 cases of outpatient physical examination healthy people at the same period were enrolled into control group.The genotypes of TNF-βA252G and C804A were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and polymerase chain reaction-sequence specific primers(PCR-SSP).Meanwhile,clinical data were collected and fasting blood glucose(FBG),total cholesterol,triglyceride,low density lipoprotein cholesterol and blood homocysteine were detected.Results The proportion of patients with hypertension,diabetes,smoking history and FBG level in CI group were significantly higher than those in control group(P<0.05).The distribution of TNF-βA252G genotype in CI group was significantly different from that in control group(P<0.05),and the GG gene frequency and G allele gene frequency were significantly higher than those in control group(P<0.05).The distribution of TNF-βC804A genotype in CI group was significantly different from that in control group(P<0.05),and the AA gene frequency and A allele gene frequency were significantly higher than those in control group(P<0.05).Multivariate Logistic regression analysis showed that hypertension,diabetes mellitus,smoking history,FBG level,TNF-βA252 genotype with GG,TNF-βA252G allele gene with G,TNF-βC804A genotype with AA,TNF-β804A allele gene with G were independent risk factors for CI(P<0.05).Conclusion TNF-βA252G genotype is GG,TNF-β252G allele gene is G,TNF-βC804A genotype is AA,TNF-βC804A allele is A may be important susceptible genes to LAA CI.

关 键 词：脑梗死 大动脉粥样硬化 肿瘤坏死因子-Β 基因多态性 

分 类 号：R743[医药卫生—神经病学与精神病学]